|Calculated MW||154197 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Con A stimulated bovine lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Antigen WC1.1, WC11|
|Target/Specificity||Mouse anti-bovine WC1, clone CC101 recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (MacHughet al.1993). The bovine WC1 cell surface antigen is expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns (Crockeret al.1993).Mouse anti-bovine WC1, clone CC101 immunoprecipitates a 215 kDa molecule from bovine cells, and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carret al.1994).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Bovine WC1 Antibody, clone CC101 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes.Immunology 81: 36-40. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattleVet. Immunol. Immunopathol. 39: 25-48. 3. MacHugh, N. et al. (1993) Clustering of monoclonal antibodies recognizing different members of the WC1 gene family.Vet Immunol Immunopathol. 39:155-60. 4. Crocker, G. et al. (1993) Analysis of the gamma/delta T cell restricted antigen WC1.Vet Immunol Immunopathol. ;39:137-44. 5. Lund, B. et al. (1993) Expression of T19 (WC1) molecules by ovine lymphocytes.Vet Immunol Immunopathol. 39:145-53. 6. Schröder, A.C. & Hamann, J. (2005) The influence of technical factors on differential cell count in milk.J Dairy Res. 72 (2): 153-8. 7. Liu, X. et al. (2014) Crusted scabies is associated with increased IL-17 secretion by skin T cells.Parasite Immunol. 36 (11): 594-604. 8. Patarroyo JH et al. (2009) Immune response of bovines stimulated by synthetic vaccine SBm7462 against Rhipicephalus (Boophilus) microplus.Vet Parasitol. 166 (3-4): 333-9. 9. Heiser A et al. (2015) Grazing dairy cows had decreased interferon-?, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving.J Dairy Sci. 98 (2): 937-46. 10. Takamatsu, H.H. et al. (2006) Porcine gammadelta T cells: possible roles on the innate and adaptive immune responses following virus infection.Vet Immunol Immunopathol. 112 (1-2): 49-61. 11. Sedlak, C. et al. (2014) IL-12 and IL-18 induce interferon-? production and de novo CD2 expression in porcine ?d T cells.Dev Comp Immunol. 47 (1): 115-22.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.2. Wijngaard, P. et al. (1992) Molecular characterization of the WC1 antigen expressed specifically on bovine CD4-CD8- gamma delta T lymphocytes.J Immunol. 149:3273-7.
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