|Application ||WB, IHC-P, IHC-F, E, IP|
|Calculated MW||43653 Da|
|Other Species||B,Cat,Hs,Green Pr|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant human p53.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53|
|Target/Specificity||Mouse anti-Human p53 antibody, clone DO-1 recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kernet al.1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Hauptet al.2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger andFersht 2007)Mouse anti-Human p53 antibody, clone DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25,common to isoforms 1-3 of p53.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-p53 (aa20-25) Antibody, clone DO-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Vojtěsek B et al. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.J Immunol Methods. 151 (1-2): 237-44. 2. Sironi, G. et al. (1999) p53 protein expression in conjunctival squamous cell carcinomas of domestic animals.Vet Ophthalmol. 2 (4): 227-231. 3. Levesque, M.A. et al. (1995) Time-resolved immunofluorometric assay of p53 protein.Clin Chem. 41 (12 Pt 1): 1720-9. 4. Bonsing, B.A. et al. (1997) Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry.Cytometry. 28 (1): 11-24. 5. Carvalho, T. et al. (2009) Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria.Vet Pathol. 46 (2): 211-21. 6. Phillips, A. et al. (2010) HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity.J Biol Chem. 285 (38): 29111-27. 7. Bergman, L.M. et al. (2009) CtBPs promote cell survival through the maintenance of mitotic fidelity.Mol Cell Biol. 29: 4539-51. 8. Hietanen, S. et al. (2000) Activation of p53 in cervical carcinoma cells by small molecules.Proc Natl Acad Sci U S A. 97 (15): 8501-6. 9. Phelps, M. et al. (2003) p53-independent activation of the hdm2-P2 promoter through multiple transcription factor response elements results in elevated hdm2 expression in estrogen receptor alpha-positive breast cancer cells.Cancer Res. 63: 2616-23.
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