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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-p53 (aa20-25) Antibody, clone DO-7    

Anti-p53 (aa20-25) Antibody, clone DO-7

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti-p53 (aa20-25) Antibody, clone DO-7  ABD12099
    Western blot analysis of HEK293 cell lysate probed with Mouse anti p53 (aa20-25) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence
  • IHC-P - Anti-p53 (aa20-25) Antibody, clone DO-7  ABD12099
    Formalin fixed, paraffin processed human breast carcinoma stained with Mouse anti p53
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04637
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names DO-7
Calculated MW 43653 Da
Additional Information
Other Species B
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant human p53.
Shelf Life 18 months from date of despatch.
Gene ID 7157
Other Names Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Target/Specificity Mouse anti-p53 antibody, clone DO-7 recognizes the human 53kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization domain and a terminal regulatory domain (Joergeret al.2010,Baiet al.2006).p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis (Hauptet al.2003) and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals (Takagiet al.2005). Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and 14-3-3σ, DNA repair and potentially apoptosis through interaction with Bax,Apaf-1,PUMAandNoxA(Thakuret al.2010). p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent system (Moll and Petrenko 2003)Mouse anti-p53 antibody, clone DO-7 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple isoforms lacking the N-teminal region.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-p53 (aa20-25) Antibody, clone DO-7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Vojtesek, B. et al. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.
J. Immunol. Methods 151: 237-24 2. Bonsing, B. A. et al (1997) Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry.
Cytometry. 28:11-24. 3. Huang, H.Y. et al. (2008) Immunohistochemical and biogenetic features of diffuse-type tenosynovial giant cell tumors: the potential roles of cyclin A, P53, and deletion of 15q in sarcomatous transformation.
Clin Cancer Res. 14: 6023-32. 4. Iannone, F. et al. (2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.
Ann Rheum Dis. 64: 217-21. 5. Xinarianos, G. et al. (2002) p53 status correlates with the differential expression of the DNA mismatch repair protein MSH2 in non-small cell lung carcinoma.
Int J Cancer. 101: 248-52. 6. Lin, L.C. et al. (20006) p53 and p27 as predictors of clinical outcome for rectal-cancer patients receiving neoadjuvant therapy.
Surg Oncol. 15: 211-6.

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$ 311.00
Cat# ABD12099
2 Formats Available
Availability: 5-7days
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