|Application ||WB, IHC-P, IHC-F, FC|
|Calculated MW||81538 Da|
|Immunogen||Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.|
|Shelf Life||18 months from date of despatch.|
|Other Names||CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4|
|Target/Specificity||Mouse anti-Human CD44v4 antibody, clone VFF-11 specifically recognizes an epitope encoded by exon v4 on the variant portion of human CD44. CD44v4 is strongly expressed on some breast cancer cell lines, notably epithelial-like BT-20 cells where v4 containing isoforms posess functional E-selectin ligand activity mediating cell adhesion under physiological flow conditions (Shirureet al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD44v4 Antibody, clone VFF-11 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.J Exp Med. 177 (4): 897-904. 2. Hanley, W.D. et al. (2005) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.FASEB J. 20: 337-9. 3. Rajarajan, A. et al. (2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.PLoS One. 7: e28776. 4. Chandrasekaran, S. et al. (2012) Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell linesBiomaterials. 33: 9037-48. 5. El-Sharkawy, M.M. et al. (2003) CD44 expression and soluble CD44 as a potential marker of tumor load in pediatric acute leukemia.J Egypt Nat Cancer Inst. 15: 129-35. 6. Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.Am J Physiol Cell Physiol. 308 (1): C68-78. 7. Hudson, D.L. et al. (1995) CD44 is the major peanut lectin-binding glycoprotein of human epidermal keratinocytes and plays a role in intercellular adhesion.J Cell Sci. 108: 1959-70. 8. De Sousa, P. A. (2009) Method for differentiation of stem cells.U.S. Patent Application: US20090123430 A1
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