|Application ||FC, IP|
|Calculated MW||82536 Da|
|Other Species||Cynomolgus Pr,Rh|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Shelf Life||18 months from date of despatch.|
|Other Names||Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1|
|Target/Specificity||Mouse anti-Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Mulleret al.1989).CD31 is also known as PECAM-1.The binding epitope for mouse anti-human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcettet al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD31 Antibody, clone WM59 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Carbanas. C., Sanchez-Madrid, F., et al. (1989) Characterisation of a novel myeloid antigen regulated during differentiation of monocytic cells.Eur J. Immunol. 19: 1373-1378. 2. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. 3. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.J. Immunol. 145: 3889-3897. 4. DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.Immunol. Today 15: 490-495. 5. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.J Pharmacol Exp Ther 321: 656-662. 6. Fawcett, J. et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31adhesion in the formation of interendothelial cell contacts.J Cell Biol. 128:1229-41. 7. Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.J Leukoc Biol. 82: 1278-88. 8. Johnston, A. et al. (2005) The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.Clin Immunol. 114: 154-63. 9. Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.J Cell Biol. 148: 1151-8. 10. Stein, A. et al. (2010) Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction.BMC Cardiovasc Disord. Sep; 10:43. 11. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.Clin Exp Immunol. 130: 256-62. 12. Theberge, A.B. et al. (2015) Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.Anal Chem. 87 (6): 3239-46. 13. Hilbe W et al. (2004) CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.J Clin Pathol. 57 (9): 965-9. 14. Hilbe W et al. (2003) Immunohistochemical typing of non-small cell lung cancer on cryostat sections: correlation with clinical parameters and prognosis.J Clin Pathol. 56 (10): 736-41. 15. Palakkan, A.A. et al. (2015) Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells.Biomed Rep. 3 (5): 626-636. 16. Newey SE et al. (2014) The hematopoietic chemokine CXCL12 promotes integration of human endothelial colony forming cell-derived cells into immature vessel networks.Stem Cells Dev. 23 (22): 2730-43. 17. Fabre-Mersseman V et al. (2011) CD4? recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis.AIDS. 25 (9): 1153-62. 18. Patten PE et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.Blood. 111 (10): 5173-81. 19. Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.J Immunol. 173 (1): 230-5. 20. Pfisterer K et al. (2015) CD90(+) human dermal stromal cells are potent inducers of FoxP3(+) regulatory T cells.J Invest Dermatol. 135 (1): 130-41. 21. Hale, S.J. et al. (2015) CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.Br J Haematol. 169 (4): 552-64. 22. Muthana, M. et al. (2015) Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting.Nat Commun. 6: 8009. 23. Schuster, C. et al. (2015) Development of Blood and Lymphatic Endothelial Cells in Embryonic and Fetal Human Skin.Am J Pathol. 185 (9): 2563-74. 24. Somers, E. et al. (2016) Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.Ann Neurol. 79 (2): 217-30.
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