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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD31 Antibody, clone WM59 (RPE)   

Anti-Human CD31 Antibody, clone WM59

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD31 Antibody, clone WM59  ABD12105
    Staining of human peripheral blood platelets with Mouse anti Human CD31:FITC
  • FC - Anti-Human CD31 Antibody, clone WM59  ABD12105
    Published customer image:Mouse anti Human CD31 antibody, clone WM59 used for the detection of CD31 positive cells by flow cytometry.Image caption:Characterisation of clonal perivascular MSC progenitor lines. (A) Single cell in vitro a few hours after plating, and culture at 7 and 21 days, respectively. Scale bar 50 µm. (B) Clonally derived MSC show the same antigen profile as detected for polyclonal lines with pericyte and MSC markers but neither neuronal nor glial precursor cell markers (O4, GFAP) (example here cortical clone; green = isotype, red = respective marker).(C) QPCR comparing clones derived from the ventricular zone (n = 2), and the cortex (n = 2) to a human neuronal progenitor cell line MesC2.10. Data show the relative higher expression of mRNA for RGS5, PDGFR-ß and a-SMA in the MSC clones compared to the control. As expected, Nestin mRNA was present in perivascular MSC clones and neural progenitors at comparable amounts. NG2 mRNA was slightly higher expressed in the MSC clones compared to the control. (D) Importantly, neural progenitors markers such as CD133, SOX1, NGN2, PAX6 and Musashi were highly expressed in the positive control but not detectable in MSC clones.From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells. PLoS ONE 7(4): e35577.
  • FC - Anti-Human CD31 Antibody, clone WM59  ABD12105
    Staining of human peripheral blood platelets with Mouse anti Human CD31:Biotin
  • FC - Anti-Human CD31 Antibody, clone WM59  ABD12105
    Published customer image:Mouse anti Human CD31 monoclonal antibody, clone WM59 used for the detection of CD31 expressing cells in cardiovacular procegitor cell cultures by immunofluorescence.Image caption:In vitro differentiation of ISL1+ CVP clones into cardiomyocytes, as well as endothelial and smooth muscle cells. Undifferentiated ISL1+ CVP clones were maintained in medium supplemented with EDN1 and WNT3A. Differentiation of the CVPs into various cardiac lineages was achieved using protocols described under material and method. (a) Immunocytochemistry demonstrating the presence of endothelial cell (EC; CD31+), smooth muscle cell (SM; SMMHC+) and cardiomyocyte (CM; CTNT+) on differentiation with various differentiation media. EC was stained with mouse anti-CD31 primary antibody while SM and CM were stained with mouse anti-SMMHC and mouse anti-CTNT, respectively, at 1:100 dilutions. Cell nuclei were stained with 4,6-diamindino-2-phenylindole . Scale bar, 100?μm. (b) Graphical representation illustrating differentiation efficiencies of ISL1+ CVP into various cardiac lineages. Flow cytometric analyses for EC (CD31+), SM (SMMHC+) and CM (CTNT+) differentiation were performed on two progenitor clones (clones 1 and 2). Bars, s.d. of n=3 experiments. *P<0.05 and **P<0.001, evaluated by Student's t-test. (c–e) Quantitative PCR confirming the expression of CD31 and VWF (EC markers); SM22 and SMMHC (SM markers); and NKX2.5, GATA4, MEF2C, TBX5, CX43 and TNNT2 (CM markers) when ISL1+ CVP clones were differentiated into the various cardiac lineages. Gene expressions were normalized to the respective undifferentiated ISL1+ CVP clones. n=3 experiments. *P<0.05 and **P<0.01, evaluated by Student's t-test.From: Soh BS, Ng SY, Wu H, Buac K, Park JH, Lian X, Xu J, Foo KS, Felldin U, He X, Nichane M, Yang H, Bu L, Li RA, Lim B, Chien KR.Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.Nat Commun. 2016 Mar 8;7:10774.
  • IF - Anti-Human CD31 Antibody, clone WM59  ABD12105
    Published customer image:Mouse anti Human CD31 monoclonal antibody, clone WM59 used for the detection of CD31 expressing lung endothelium by immunofluorescenceImage caption:Figure 7. Apigenin reduces LPS-induced apoptosis in the lungs. Lungs from mice treated with LPS, control, apigenin or apigenin 3 h prior to LPS (Api + LPS) were obtained at 24 h. (A) The number of apoptotic cells was determined by the TUNEL assay and expressed as the mean ± SEM of the numbers of stained cells per 1000× microscopic field (N = 4 mice for each biological condition; * p < 0.01 vs. LPS), scale bars: 25 μm for all figures; (B,C) Caspase-3 activity was determined by the DEVD-AFC assay using lung lysates as described in materials and methods. Values represent the means ± SEM (N = 4 mice for each biological condition; * p < 0.05 vs. LPS); (D) Lung tissue immuno-histochemistry using antibodies specific for active caspase-3 in combination with anti-CD31, anti-cytokeratin or anti-7/4 antibodies specific for endothelia, epithelia and neutrophils, respectively. Scale bars, 100 μm.From: Cardenas, H.; Arango, D.; Nicholas, C.; Duarte, S.; Nuovo, G.J.; He, W.; Voss, O.H.; Gonzalez-Mejia, M.E.; Guttridge, D.C.; Grotewold, E.; Doseff, A.I.Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.Int. J. Mol. Sci. 2016, 17, 323.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16284
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names WM59
Calculated MW 82536 Da
Additional Information
Other Species Cynomolgus Pr,Rh
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Gene ID 5175
Other Names Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
Target/Specificity Mouse anti-Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Mulleret al.1989).CD31 is also known as PECAM-1.The binding epitope for mouse anti-human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcettet al.1995).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD31 Antibody, clone WM59 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Carbanas. C., Sanchez-Madrid, F., et al. (1989) Characterisation of a novel myeloid antigen regulated during differentiation of monocytic cells.
Eur J. Immunol. 19: 1373-1378. 2. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. 3. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.
J. Immunol. 145: 3889-3897. 4. DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.
Immunol. Today 15: 490-495. 5. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther 321: 656-662. 6. Fawcett, J. et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31adhesion in the formation of interendothelial cell contacts.
J Cell Biol. 128:1229-41. 7. Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.
J Leukoc Biol. 82: 1278-88. 8. Johnston, A. et al. (2005) The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.
Clin Immunol. 114: 154-63. 9. Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.
J Cell Biol. 148: 1151-8. 10. Stein, A. et al. (2010) Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction.
BMC Cardiovasc Disord. Sep; 10:43. 11. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
Clin Exp Immunol. 130: 256-62. 12. Theberge, A.B. et al. (2015) Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.
Anal Chem. 87 (6): 3239-46. 13. Hilbe W et al. (2004) CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.
J Clin Pathol. 57 (9): 965-9. 14. Hilbe W et al. (2003) Immunohistochemical typing of non-small cell lung cancer on cryostat sections: correlation with clinical parameters and prognosis.
J Clin Pathol. 56 (10): 736-41. 15. Palakkan, A.A. et al. (2015) Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells.
Biomed Rep. 3 (5): 626-636. 16. Newey SE et al. (2014) The hematopoietic chemokine CXCL12 promotes integration of human endothelial colony forming cell-derived cells into immature vessel networks.
Stem Cells Dev. 23 (22): 2730-43. 17. Fabre-Mersseman V et al. (2011) CD4? recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis.
AIDS. 25 (9): 1153-62. 18. Patten PE et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.
Blood. 111 (10): 5173-81. 19. Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.
J Immunol. 173 (1): 230-5. 20. Pfisterer K et al. (2015) CD90(+) human dermal stromal cells are potent inducers of FoxP3(+) regulatory T cells.
J Invest Dermatol. 135 (1): 130-41. 21. Hale, S.J. et al. (2015) CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.
Br J Haematol. 169 (4): 552-64. 22. Muthana, M. et al. (2015) Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting.
Nat Commun. 6: 8009. 23. Schuster, C. et al. (2015) Development of Blood and Lymphatic Endothelial Cells in Embryonic and Fetal Human Skin.
Am J Pathol. 185 (9): 2563-74. 24. Somers, E. et al. (2016) Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.
Ann Neurol. 79 (2): 217-30.

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Cat# ABD12105
(40 western blots)
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