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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human CD227 Antibody, clone C595 (NCRC48) (FITC)   

Anti-Human CD227 Antibody, clone C595 (NCRC48)

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti-Human CD227 Antibody, clone C595 (NCRC48)  ABD12106
    Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 as a detection reagent with purified breast cancer antigen (BioRad Catalog 22502313) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
  • IF - Anti-Human CD227 Antibody, clone C595 (NCRC48)  ABD12106
    Immunofluorescence staining of MCF-7 cells with Mouse anti Human CD227:FITC , nuclei are counterstained with DAPI
  • E - Anti-Human CD227 Antibody, clone C595 (NCRC48)  ABD12106
    Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, FC, E
Primary Accession P15941
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG3
Clone Names C595 (NCRC48)
Calculated MW 122102 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Urinary MUC-1 mucin.
Shelf Life 18 months from date of despatch.
Gene ID 4582
Other Names Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM
Target/Specificity Mouse anti-Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti-Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD227 Antibody, clone C595 (NCRC48) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Price, M.R. et al. (1990) Immunological and structural features of the protein core of human polymorphic epithelial mucin.
Mol Immunol. 27 (8): 795-802. 2. Price, M.R. et al. (1990) C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas.
Br J Cancer. 61 (5): 681-6. 3. Price, M.R. et al. (1991) Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography.
J Immunol Methods. 139 (1): 83-90. 4. Denton, G. et al. (1995) Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody.
Eur J Cancer. 31A (2): 214-21. 5. Denton, G. et al. (1997) Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas.
Br J Cancer. 76 (5): 614-21. 6. Murray, A. et al (2001) Production and characterization of 188Re-C595 antibody for radioimmunotherapy of transitional cell bladder cancer.
J Nucl Med. 42: 726-32. 7. Hisatsune, A. et al. (2011) Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.
Biochem Biophys Res Commun. 405: 377-81. 8. Ragupathi, G. et al. (2005) Antibodies against tumor cell glycolipids and proteins, but not mucins, mediate complement-dependent cytotoxicity.
J Immunol. 174: 5706-12. 9. Huang, D.M. et al. (2004) Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells.
Prostate. 2004 May 15;59(3): 260-7.

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