|Application ||WB, IHC-P, IHC-F, IF, FC, E|
|Clone Names||C595 (NCRC48)|
|Calculated MW||122102 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Urinary MUC-1 mucin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM|
|Target/Specificity||Mouse anti-Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti-Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD227 Antibody, clone C595 (NCRC48) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Price, M.R. et al. (1990) Immunological and structural features of the protein core of human polymorphic epithelial mucin.Mol Immunol. 27 (8): 795-802. 2. Price, M.R. et al. (1990) C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas.Br J Cancer. 61 (5): 681-6. 3. Price, M.R. et al. (1991) Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography.J Immunol Methods. 139 (1): 83-90. 4. Denton, G. et al. (1995) Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody.Eur J Cancer. 31A (2): 214-21. 5. Denton, G. et al. (1997) Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas.Br J Cancer. 76 (5): 614-21. 6. Murray, A. et al (2001) Production and characterization of 188Re-C595 antibody for radioimmunotherapy of transitional cell bladder cancer.J Nucl Med. 42: 726-32. 7. Hisatsune, A. et al. (2011) Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.Biochem Biophys Res Commun. 405: 377-81. 8. Ragupathi, G. et al. (2005) Antibodies against tumor cell glycolipids and proteins, but not mucins, mediate complement-dependent cytotoxicity.J Immunol. 174: 5706-12. 9. Huang, D.M. et al. (2004) Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells.Prostate. 2004 May 15;59(3): 260-7.
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