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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD66e Antibody, clone C365D3(NCRC23) (FITC)   

Anti-Human CD66e Antibody, clone C365D3(NCRC23)

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD66e Antibody, clone C365D3(NCRC23)  ABD12108
    Published customer image:Immunostaining and cell surface expression of epithelial/pancreatic markers and overexpressed proteins in PaCa 5061 cells. 2A. The formaldehyde fixed and agar embedded cells were immunostained for the presence of pancreatic cancer markers CA 19-9 and CEA respectively, as well as for cytokeratin as epithelial cell marker proteins (upper panel). Several on RNA-level overexpressed genes (Microarray) were chosen. To confirm protein overexpression of selected amplified genes in PaCa 5061 cells immunostaining was performed for Mucin4, EGFR and CEACAM 6 (lower panel). 2B. FACS profiles of PaCa 5061 cells. Cell surface expression of CD44, EpCAM, EGFR, CEACAM1, CEACAM5 and CEACAM6 were obtained with specific antibodies as in materials and methods. Each histogram shows cell surface expression of the corresponding marker (filled curves) and the irrelevant, isotype-matched antibody (open curves). From: Kalinina T, Güngör C, Thieltges S, Möller-Krull M, Penas EM, Wicklein D, Streichert T, Schumacher U, Kalinin V, Simon R, Otto B, Dierlamm J, Schwarzenbach H, Effenberger KE, Bockhorn M, Izbicki JR, Yekebas EF. Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung. BMC Cancer. 2010 Jun 16;10:295.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06731
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names C365D3(NCRC23)
Calculated MW 76795 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Gene ID 1048
Other Names Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e, CEACAM5, CEA
Target/Specificity Mouse anti-Human CD66e antibody, clone C365D3 (NCRC23) recognizes human Carcinoembryonic antigen-related cell adhesion molecule 5, also known as CD66e, carcinoembryonic antigen, Meconium antigen 100, CEA or CEACAM5. CD66e is a 702 amino acid ~77 kDa GPI anchored membrane protein containing 7 Ig-like domains. Mouse anti-Human CD66e antibody, clone C365D3 does not cross-react with normal cross-reacting antigen (CD66c), or with biliary g lycoprotein (CD66a) as indicated by binding assays (Price 1988, note: in this study Mouse anti-Human CD66e antibody, clone C365D3 is designated as clone 6 (from author)).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD66e Antibody, clone C365D3(NCRC23) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Price, M.R. (1988) Epitope of carcinoembryonic antigen (CEA) defined by monoclonal antibodies.
Br. J. Cancer 57: 165-169. 2. Soucek, K. et al. (2010) Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.
Cancer Genet Cytogenet. 197: 107-16. 3. Kalinina, T. et al. (2010) Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.
BMC Cancer.10: 295. 4. Dallas, M.R. et al. (2012) Divergent roles of CD44 and carcinoembryonic antigen in colon cancer metastasis.
FASEB J. 226: 2648-56. 5. Stern-Ginossar. N. et al. (2007) Intercellular Transfer of Carcinoembryonic Antigen from Tumor Cells to NK Cells.
J Immunol. 2007 Oct 1;1 79: 4424-34. 6. Ferro, F. et al. (2011) Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure.
Am J Pathol.178: 2299-310. 7. Chao, A. et al. (2006) Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression.
Int J Cancer. 119: 91-8.

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Cat# ABD12108
(40 western blots)
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