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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Pig CD31 Antibody, clone LCI-4 (RPE)   

Anti-Pig CD31 Antibody, clone LCI-4 (APC)

Mouse Anti-Pig Monoclonal Antibody

     
  • IHC - Anti-Pig CD31 Antibody, clone LCI-4 (APC) ABD12109
    Published customer image:Mouse anti Pig CD31 antibody, clone LCI-4 used for the detection of blood vessels in normal and diabetic human patient skin by immunohistochemistery of formalin fixed, paraffin embedded tissue sections.Image caption:Similar numbers of lymphocytes, blood vessels and proliferating keratinocytes are found in DFS and NFS. Immunostaining and quantification for CD45, a lymphocyte marker, (A, D), CD31, an endothelial cell marker, (B, E) and Ki67, a proliferation marker (C,F), reveal no differences in the number of lymphocytes, blood vessels and proliferating keratinocytes found in DFS and NFS. Ki67 immuno-peroxidase staining for proliferating cells (C) shows that proliferative keratinocytes were located in the basal layer of the skin, as expected. No significant differences were found between DFS and NFS in numbers of CD45+ cells (D), number of CD31+ (E) or Ki67 positive keratinocytes (F). Bar graphs indicate mean and SD.From: Ramirez HA, Liang L, Pastar I, Rosa AM, Stojadinovic O, Zwick TG, et al. Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin.PLoS ONE 10: e0137133.
  • IF - Anti-Pig CD31 Antibody, clone LCI-4 (APC) ABD12109
    Published customer image:Mouse anti Pig CD31 antibody, clone LCI-4 used for the detection of vascular endothelium in pigs by immunofluorescence.Image caption:VECs produce, release, and actively cleave von Willebrand factor. (A) Non-stimulated VECs seeded on hydrogels (3.4 kDa) functionalized with RKR or RGDS stained for CD31 and VWF . Nucleus was stained with DAPI . Scale bar = 50 &mu;m. (B) Quantification of rapidly released VWF and (C) the fraction of cleaved VWF-140 fragments from histamine-stimulated VECs cultured on different molecular weight hydrogels functionalized with RKR or RGDS, or TCPS. Groups not connected by same symbols are significantly different. p < 0.05.From: Balaoing LR, Post AD, Lin AY, Tseng H, Moake JL, Grande-Allen KJ Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.PLoS ONE 10(6): e0130749.
  • FC - Anti-Pig CD31 Antibody, clone LCI-4 (APC) ABD12109
    Published customer image:Mouse anti Pig CD31 antibody, clone LCI-4 used for the evaluation of CD31 expression on porcine differentiated fat cells by flow cytometry.Image caption:Expression of cell surface antigens on porcine DFAT cells. Expressions of CD44, CD29, CD90, CD31, and CD34 were detected by FITC or CY3 conjugated antibody of P4 DFAT cells.From: Peng X, Song T, Hu X, Zhou Y, Wei H, Peng J, Jiang S. Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro. Biomed Res Int. 2015;2015:673651.
  • FC - Anti-Pig CD31 Antibody, clone LCI-4 (APC) ABD12109
    Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD31:FITC
  • FC - Anti-Pig CD31 Antibody, clone LCI-4 (APC) ABD12109
    Published customer image:Mouse anti Pig CD31 antibody, clone LCI-4 used for the evaluation of PECAM-1 expression on M. suis infected porcine endothelial cells.Image caption:CAM expression in M. suis infected endothelial cells. The expression profiles of adhesion molecules on endothelial cells were analyzed using a FACSCanto II cell sorting system. A. Dashed lines represent the isotype control; solid lines represent cells incubated with the negative control preparation; grey histograms represent PEDSV.15 cells incubated with M. suis (1?×?104 cells/mL). B. Percent increase in expression ICAM-1 , PECAM-1 , E - and P -selectin in ECs infected with M. suis.From: Sokoli A, Groebel K, Hoelzle K, Amselgruber WM, Mateos JM, Schneider MK, Ziegler U, Felder KM, Hoelzle LE. Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma.Vet Res. 2013 Feb 11;44:6.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession Q95242
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names LCI-4
Calculated MW 82379 Da
Additional Information
Other Species H
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Porcine CD31/human IgGFc fusion protein.
Shelf Life 18 months from date of despatch.
Gene ID 396941
Other Names Platelet endothelial cell adhesion molecule, PECAM-1, CD31, PECAM1
Target/Specificity Mouse anti-Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwettet al.1995). The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmambrane region as demonstrated by human CD31 domain deletion mutant protein binding studies (Nasuet al.1999).Mouse anti-Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasuet al.1999).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; 5% Sucrose
Storage Prior to reconstitution store at +4℃.
PrecautionsAnti-Pig CD31 Antibody, clone LCI-4 (APC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
Transplantation. 68: 861-7. 2. Evans, P.C. et al. (2001) Signaling through CD31 protects endothelial cells from apoptosis.
Transplantation. 71 (3): 343-4. 3. Gesslein, B. et al. (2010) Mitogen-activated protein kinases in the porcine retinal arteries and neuroretinafollowing retinal ischemia-reperfusion.
Mol Vis. 16: 392-407. 4. Gyöngyösi, M. et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
Thromb Haemost. 103: 1-9. 5. Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
Stem Cells. 26: 2408-2418. 6. Campos, E. et al. (2004) In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line
Vet Res. 35: 625-33. 7. Gyöngyösi, M. et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
Thromb Haemost. 2010 Aug;104(2):376-84. 8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
Stem Cells. 26: 1347-55. 9. Poirier, N. et al. (2010) Inducing CTLA-4-dependent immune regulation by selective CD28 blockade promotes regulatory T cells in organ transplantation.
Sci Transl Med. 2: 17ra10. 10. Tchorsh-Yutsis, D. et al. (2009) Pig embryonic pancreatic tissue as a source for transplantation in diabetes: transient treatment with anti-LFA1, anti-CD48, and FTY720 enables long-term graft maintenance in mice with only mild ongoing immunosuppression.
Diabetes. 58: 1585-94. 11. Waksman, R. et al. (2006) Intracoronary photodynamic therapy reduces neointimal growth without suppressing re-endothelialisation in a porcine model.
Heart. 92: 1138-44. 12. Chatelais, M. et al. (2011) Gene transfer of the adaptor Lnk (SH2B3) prevents porcine endothelial cell activation and apoptosis: implication for xenograft's cytoprotection.
Xenotransplantation. 18: 108-20. 14. Chitalia, V.C. et al. (2011) Matrix-embedded endothelial cells are protected from the uremic milieu.
Nephrol Dial Transplant. 26: 3858-65. 15. Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Ann Biomed Eng. 41 (10): 2181-92. 16. Graham, J.J. et al. (2010) Long-term tracking of bone marrow progenitor cells following intracoronary injection post-myocardial infarction in swine using MRI.
Am J Physiol Heart Circ Physiol. 299: H125-33. 17. Azimzadeh, A.M. et al. (2014) Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells.
Xenotransplantation. 21 (6): 555-66. 18. Andrée, B. et al. (2014) Successful re-endothelialization of a perfusable biological vascularized matrix (BioVaM) for the generation of 3D artificial cardiac tissue.
Basic Res Cardiol. 109: 441. 19. Sokoli, .A. et al.(2013)Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma.
Vet Res.44: 6. 20. Takeda, S. et al. (2006) Differential origin for endothelial and mesangial cells after transplantation of pig fetal renal primordia into rats.
Transpl Immunol. 15: 211-5. 21. Balaoing, L.R. et al. (2015) Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.
PLoS One. 10 (6): e0130749. 22. Ramirez, H.A. et al. (2015) Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin.
PLoS One. 10 (8): e0137133. 23. Leitão AF et al. (2015) A Novel Small-Caliber Bacterial Cellulose Vascular Prosthesis: Production, Characterization, and Preliminary In VivoTesting.
Macromol Biosci. Sep 21. [Epub ahead of print] 24. Zhang, Q. et al. (2015) Engineering vascularized soft tissue flaps in an animal model using human adipose-derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle.
Biomaterials. 73: 198-213. 25. Puperi, D.S. et al. (2015) 3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.
Biomaterials. 67: 354-64. 26. Barsotti, M.C. et al. (2015) Oligonucleotide biofunctionalization enhances endothelial progenitor cell adhesion on cobalt/chromium stents.
J Biomed Mater Res A. 103 (10): 3284-92. 27. Peng X et al. (2015) Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro.
Biomed Res Int. 2015: 673651.13. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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