|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Leucocytes isolated from porcine gut lamina propria.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Porcine CD4 alpha, clone MIL17 recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haversonet al.2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmulleret al.1987)Mouse anti-Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inmanet al.2010).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD4 alpha Antibody, clone MIL17 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.Vet Immunol Immunopathol. 80 (1-2): 35-52. 2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage. Am J Pathol. 176: 1648-59. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88 (3): 571-8. 4. Kick AR et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.Res Vet Sci. 91 (3): e68-72. 5. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.J Anim Sci. 90: 649-56. 6. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.Kidney Int. 58: 838-50. 7. Tambuyzer BR et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.Cell Biol Int. 36 (12): 1233-8. 8. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.BMC Vet Res. 8: 232. 9. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.Vaccine. 28: 6006-13. 10. Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigsLivestock Production Science 96: 249-60 11. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.Vet Immunol Immunopathol. 104: 131-44. 12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.Animal. 2: 1575-84. 13. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.J Pharmacol Exp Ther. 2002 Sep;302(3):861-70. 14. Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model.Nephrol Dial Transplant. 19: 1742-51. 15. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.Kidney Int. 62: 654-67. 16. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.J Anim Sci. 90: 649-56. 17. Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus.Eur J Clin Microbiol Infect Dis. 29: 1453-6. 18. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.BMC Genomics. 13: 488. 19. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.J Swine Health Prod 20: 276-282. 20. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.Vet Microbiol. 129: 367-77. 21. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.J Nutr. 131: 1520-7. 22. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.Cell Biol Int. 36: 1233-8. 23. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.J Immunol Methods. 327: 18-29. 24. Kvist, P.H. et al. (2010) Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs.Diabetes Technol Ther. 12: 791-9. 25. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.PLoS One. 7(3): e32400. 26. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.PLoS One. 7(3): e33707. 27. Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.PLoS One. 11 (3): e0151256.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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