|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Leucocytes isolated from porcine gut lamina propria.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Porcine CD4 alpha, clone MIL17 recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haversonet al.2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmulleret al.1987)Mouse anti-Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inmanet al.2010).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD4 alpha Antibody, clone MIL17 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.Vet Immunol Immunopathol. 80 (1-2): 35-52. 2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage. Am J Pathol. 176: 1648-59. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88 (3): 571-8. 4. Kick AR et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.Res Vet Sci. 91 (3): e68-72. 5. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.J Anim Sci. 90: 649-56. 6. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.Kidney Int. 58: 838-50. 7. Tambuyzer BR et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.Cell Biol Int. 36 (12): 1233-8. 8. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.BMC Vet Res. 8: 232. 9. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.Vaccine. 28: 6006-13. 10. Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigsLivestock Production Science 96: 249-60 11. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.Vet Immunol Immunopathol. 104: 131-44. 12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.Animal. 2: 1575-84. 13. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.J Pharmacol Exp Ther. 2002 Sep;302(3):861-70. 14. Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model.Nephrol Dial Transplant. 19: 1742-51. 15. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.Kidney Int. 62: 654-67. 16. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.J Anim Sci. 90: 649-56. 17. Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus.Eur J Clin Microbiol Infect Dis. 29: 1453-6. 18. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.BMC Genomics. 13: 488. 19. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.J Swine Health Prod 20: 276-282. 20. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.Vet Microbiol. 129: 367-77. 21. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.J Nutr. 131: 1520-7. 22. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.Cell Biol Int. 36: 1233-8. 23. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.J Immunol Methods. 327: 18-29. 24. Kvist, P.H. et al. (2010) Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs.Diabetes Technol Ther. 12: 791-9. 25. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.PLoS One. 7(3): e32400. 26. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.PLoS One. 7(3): e33707. 27. Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.PLoS One. 11 (3): e0151256.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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