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Anti-Bovine Interferon gamma Antibody, clone CC302

Mouse Anti-Bovine Monoclonal Antibody

     
  • FC - Anti-Bovine Interferon gamma Antibody, clone CC302  ABD12117
    Published customer image:Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN-γ within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN-γ and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6: e29194.
  • FC - Anti-Bovine Interferon gamma Antibody, clone CC302  ABD12117
    Published customer image:Mouse anti Bovine interferonγ antibody, clone CC302 used for the identification of interferonγ expressing dog lymphocytes by flow cytometry.Image caption:Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.
  • FC - Anti-Bovine Interferon gamma Antibody, clone CC302  ABD12117
    Published customer image:RPE conjugated mouse anti Bovine Ifn-γ antibody, clone CC302 used to evaluate ifn-γ expression in canine lymphocytes by flow cytometry.Image caption:CD4+ T cells from Ech_0660 mutant vaccinated and wild-type E. chaffeensis infected animals produce IFNγ in response to E. chaffeensis antigen. PBMC from dogs vaccinated with the Ech_0660 mutant and challenged with wild-type E. chaffeensis (groups 1–3, as in Fig 2) were cultured for 5 days at 4x106 cells/mL in the presence or absence of 10 μg/mL E. chaffeensis host-cell free lysate grown in the tick ISE6 cell line. On day 5, brefeldin A was added for the last 6 hours of culture. CD4+ T cells were stained for intracellular expression of IFNγ and analyzed by flow cytometry. (A) Representative flow plots from animals in groups 1, 2 and 3, gated on total live cells and total CD3+CD4+ T cells. (B) The percentage of IFNγ+ cells of total CD4+ T cells in the blood measured over the course of the experiment. Background (mock stimulated) IFNγ production was subtracted, and results represent change over mock.From: McGill JL, Nair ADS, Cheng C, Rusk RA, Jaworski DC, Ganta RR Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.PLoS ONE 11(2): e0148229.
  • FC - Anti-Bovine Interferon gamma Antibody, clone CC302  ABD12117
    Published customer image:Mouse antiBovine Ifn γantibody, clone CC302 and Mouse anti Bovine IL-4 antibody, clone CC303 used to evaluate cytokine expression by canine peripheral blood cells following short term in vitro stimulation and culture by flow cytometry.Image caption:Internal controls to validate the cross-reactivity of anti-human cytokine mAbs with canine cytokines following short-term whole blood cultures in vitro. Non-specific binding was monitored by using isotype matched reagents (top panels). Blocking strategy with host serum was also applied (bottom panels). Controls of reference cross-reactive mAbs (anti-bovina IFN-? and IL-4) were also included. Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel were used to quantify the percentage of cytokine+ lymphocytes in unstimulated controls (left panels) and PMA + LPS stimulated cultures (right panels). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1From: Moreira ML, Dorneles EM, Soares RP, Magalhães CP, Costa-Pereira C, Lage AP, Teixeira-Carvalho A, Martins-Filho OA, Araújo MS. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining.Acta Vet Scand. 2015 Sep 11;57:51.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, E
Primary Accession P07353
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC302
Calculated MW 19393 Da
Additional Information
Other Species H,Pig,Dog,Hs,Sh,Mustelid,Ga,Dolphin,Ferret,Mink,Fin Whale
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Gene ID 281237
Other Names Interferon gamma, BoIFNG, IFN-gamma, IFNG
Target/Specificity Mouse anti-Bovine IFNγ antibody, clone CC302, recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti-proliferitive properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodeset al.2000).Mouse anti-bovine γ inerferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersenet al.2002). Clone CC302 has been successfully used for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium.subspparatuberculosis(Appanaet al.2013) as a detection reagent using an ELISA.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine Interferon gamma Antibody, clone CC302 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Hasvold, H.J. et al.(2002)In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium aviumsubspparatuberculosis.
Vet Immunol Immunopathol. 90 (1-2): 79-89. 2. Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.
J Immunol. 169 (7): 3837-46. 3. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
Vet Immunol Immunopathol. 88 (3-4): 111-22. 4. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
Clin Diagn Lab Immunol. 9 (6): 1229-34. 5. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host.
J Gen Virol. 90: 2157-65. 6. Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.
J Comp Pathol. 143: 45-51. 7. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. 8. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24. 9. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. 10. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium aviumsubsp.paratuberculosis-infected goats.
Clin Vaccine Immunol. 16 (7): 1003-11. 11. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. 12. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.
Vet Immunol Immunopathol. 140: 175-80. 13. Pillet, S. et al. (2011) Cellular immune response in the presence of protective antibody levels correlates with protection against 1918 influenza in ferrets.
Vaccine. 29 (39): 6793-801. 14. Jensen, T.H. et al. (2009) Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper.
Vaccine. 27: 5178-83. 15. Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortusinfections.
PLoS One. 6:e21978. 16. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194. 17. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
BMC Vet Res. 11 (1): 92. 18. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. 19. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
Pol J Vet Sci. 15 (1): 11-20. 20. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.
Clin Vaccine Immunol. 20: 1669-74. 21. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 2014 Dec 16;10(1):293. 22. Köhler. H, et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection
BMC Veterinary Research. 11 (1): 74. 23. Moreira Marcela L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes
Veterinary Parasitology. 15 Feb [Epub ahead of print] 24. Rodríguez-Gómez IM et al. (2016) Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells.
Dev Comp Immunol. 60: 115-26. 25. Taylor, G. et al. (2015) Efficacy of a virus-vectored vaccine against human and bovine respiratory syncytial virus infections.
Sci Transl Med. 7 (300): 300ra127. 26. Moreira, M.L. et al. (2015) Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining.
Acta Vet Scand. 57: 51. 27. El-Naggar, M.M. et al. (2015) Development of an improved ESAT-6 and CFP-10 peptide-based cytokine flow cytometric assay for bovine tuberculosis.
Comp Immunol Microbiol Infect Dis. 42: 1-7. 28. McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
PLoS One. 11 (2): e0148229. 29. Vida, B. et al. (2016) Immunologic progression of canine leishmaniosis following vertical transmission in United States dogs.
Vet Immunol Immunopathol. 169: 34-8. 30. Totté, P. et al. (2010) CD62L defines a subset of pathogen-specific bovine CD4 with central memory cell characteristics.
Dev Comp Immunol. 34 (2): 177-82. 31. Sun L et al. (2012) The role of proliferation in the regulation of interferon gamma (IFN?) expression in foals.
Dev Comp Immunol. 36 (3): 534-9. 32. Reber, A.J. et al. (2006) Evaluation of multiple immune parameters after vaccination with modified live or killed bovine viral diarrhea virus vaccines.
Comp Immunol Microbiol Infect Dis. 29 (1): 61-77. 33. Katepalli, M.P. et al. (2008) The effect of age and telomere length on immune function in the horse.
Dev Comp Immunol. 32 (12): 1409-15. 34. Hansen, S. et al. (2013) Age-related changes in intracellular expression of IFN-γ and TNF-α in equine lymphocytes measured in bronchoalveolar lavage and peripheral blood.
Dev Comp Immunol. 39 (3): 228-33. 35. Boshra H et al. (2015) A lumpy skin disease virus deficient of an IL-10 gene homologue provides protective immunity against virulent capripoxvirus challenge in sheep and goats.
Antiviral Res. 123: 39-49. 36. Hedges, J.F. et al. (2015) Amphotericin B stimulates γδ T and NK cells, and enhances protection from Salmonellainfection.
Innate Immun. 21 (6): 598-608. 37. Johnson, W.C. et al. (2008) Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18.
Dev Comp Immunol. 32 (8): 1002-10.1. Rhodes, S. et al. (2000) Distinct response kinetics of gamma interferon and interleukin-4 in bovine tuberculosis.
Infect Immun. 68:5393-400.

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