|Application ||FC, E|
|Calculated MW||19393 Da|
|Other Species||H,Pig,Dog,Hs,Sh,Mustelid,Ga,Dolphin,Ferret,Mink,Fin Whale|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon gamma, BoIFNG, IFN-gamma, IFNG|
|Target/Specificity||Mouse anti-Bovine IFNγ antibody, clone CC302, recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti-proliferitive properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodeset al.2000).Mouse anti-bovine γ inerferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersenet al.2002). Clone CC302 has been successfully used for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium.subspparatuberculosis(Appanaet al.2013) as a detection reagent using an ELISA.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine Interferon gamma Antibody, clone CC302 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Hasvold, H.J. et al.(2002)In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium aviumsubspparatuberculosis.Vet Immunol Immunopathol. 90 (1-2): 79-89. 2. Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.J Immunol. 169 (7): 3837-46. 3. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.Vet Immunol Immunopathol. 88 (3-4): 111-22. 4. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.Clin Diagn Lab Immunol. 9 (6): 1229-34. 5. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host. J Gen Virol. 90: 2157-65. 6. Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.J Comp Pathol. 143: 45-51. 7. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15. 8. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24. 9. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19. 10. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium aviumsubsp.paratuberculosis-infected goats.Clin Vaccine Immunol. 16 (7): 1003-11. 11. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J Immunol. 185: 3313-25. 12. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.Vet Immunol Immunopathol. 140: 175-80. 13. Pillet, S. et al. (2011) Cellular immune response in the presence of protective antibody levels correlates with protection against 1918 influenza in ferrets.Vaccine. 29 (39): 6793-801. 14. Jensen, T.H. et al. (2009) Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper.Vaccine. 27: 5178-83. 15. Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortusinfections.PLoS One. 6:e21978. 16. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS One. 6: e29194. 17. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92. 18. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.Vet Immunol Immunopathol. 146: 1-7. 19. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.Pol J Vet Sci. 15 (1): 11-20. 20. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.Clin Vaccine Immunol. 20: 1669-74. 21. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res. 2014 Dec 16;10(1):293. 22. Köhler. H, et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infectionBMC Veterinary Research. 11 (1): 74. 23. Moreira Marcela L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytesVeterinary Parasitology. 15 Feb [Epub ahead of print] 24. Rodríguez-Gómez IM et al. (2016) Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells.Dev Comp Immunol. 60: 115-26. 25. Taylor, G. et al. (2015) Efficacy of a virus-vectored vaccine against human and bovine respiratory syncytial virus infections.Sci Transl Med. 7 (300): 300ra127. 26. Moreira, M.L. et al. (2015) Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining.Acta Vet Scand. 57: 51. 27. El-Naggar, M.M. et al. (2015) Development of an improved ESAT-6 and CFP-10 peptide-based cytokine flow cytometric assay for bovine tuberculosis.Comp Immunol Microbiol Infect Dis. 42: 1-7. 28. McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.PLoS One. 11 (2): e0148229. 29. Vida, B. et al. (2016) Immunologic progression of canine leishmaniosis following vertical transmission in United States dogs.Vet Immunol Immunopathol. 169: 34-8. 30. Totté, P. et al. (2010) CD62L defines a subset of pathogen-specific bovine CD4 with central memory cell characteristics.Dev Comp Immunol. 34 (2): 177-82. 31. Sun L et al. (2012) The role of proliferation in the regulation of interferon gamma (IFN?) expression in foals.Dev Comp Immunol. 36 (3): 534-9. 32. Reber, A.J. et al. (2006) Evaluation of multiple immune parameters after vaccination with modified live or killed bovine viral diarrhea virus vaccines.Comp Immunol Microbiol Infect Dis. 29 (1): 61-77. 33. Katepalli, M.P. et al. (2008) The effect of age and telomere length on immune function in the horse.Dev Comp Immunol. 32 (12): 1409-15. 34. Hansen, S. et al. (2013) Age-related changes in intracellular expression of IFN-γ and TNF-α in equine lymphocytes measured in bronchoalveolar lavage and peripheral blood.Dev Comp Immunol. 39 (3): 228-33. 35. Boshra H et al. (2015) A lumpy skin disease virus deficient of an IL-10 gene homologue provides protective immunity against virulent capripoxvirus challenge in sheep and goats.Antiviral Res. 123: 39-49. 36. Hedges, J.F. et al. (2015) Amphotericin B stimulates γδ T and NK cells, and enhances protection from Salmonellainfection.Innate Immun. 21 (6): 598-608. 37. Johnson, W.C. et al. (2008) Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18.Dev Comp Immunol. 32 (8): 1002-10.1. Rhodes, S. et al. (2000) Distinct response kinetics of gamma interferon and interleukin-4 in bovine tuberculosis.Infect Immun. 68:5393-400.
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