|Application ||IHC-P, IHC-F, FC, IP|
|Calculated MW||134277 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Extracellular domain of human EGF-receptor from head and neck carcinoma.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Epidermal growth factor receptor, 188.8.131.52, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1|
|Target/Specificity||Rat anti-Human EGF Receptor antibody, clone ICR10 recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas.High level expression of EGFR is often associated with advanced disease and poor prognosis.Rat anti-Human EGF Receptor antibody, clone ICR10 binds to epitope B from EGFR (Lottazet al.2010andModjtahediet al.1993) and has an affinity of 6.7 x 10-9M.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human EGF Receptor Antibody, clone ICR10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Lottaz,C. et al. (2010) Transcriptional Profiles of CD133+ and CD133- Glioblastoma-Derived Cancer Stem Cell Lines Suggest Different Cells of OriginCancer Res. 70: 2030-40. 2. Modjtahedi, H. et al. (1993) Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor.Cell Biophys. 1993. 22 (1-3): 129-146. 3. Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.Br J Cancer. 106: 883-888 4. Grinberg, O. et al. (2013) Antibody modified Bovine Serum Albumin microspheres for targeted delivery of anticancer agent GemcitabinePolymers for Advanced Technologies. 24 (3): 294-299. 5. Gilcrease, M.Z. et al. (2009) Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer.J Exp Clin Cancer Res. 28: 67. 6. Lottaz, C. et al. (2010) Transcriptional profiles of CD133+ and CD133- glioblastoma-derived cancer stem cell lines suggest different cells of origin.Cancer Res. 70: 2030-40. 7. Tilburgs, T. et al. (2015) Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes.Proc Natl Acad Sci U S A. 112 (23): 7219-24.
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