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Anti-Bovine Interleukin-4 Antibody, clone CC303

Mouse Anti-Bovine Monoclonal Antibody

     
  • FC - Anti-Bovine Interleukin-4 Antibody, clone CC303  ABD12120
    Published customer image:Mouse anti Bovine interleukin-4 antibody used for the detection of IL-4 expressing cells in yellow cattle and water buffalo following schistosome infection by flow cytometry.Image caption:Percentage of IFN-? and IL-4 positive cells detected by flow cytometry pre and post infection. yellow cattle; (B) water buffalo. Level of significance when compared to water buffalo group at the same time, *p < 0.05.Yang J, Fu Z, Feng X, Shi Y, Yuan C, Liu J, Hong Y, Li H, Lu K, Lin J. Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo. BMC Vet Res. 2012 Mar 13;8:25.
  • FC - Anti-Bovine Interleukin-4 Antibody, clone CC303  ABD12120
    Published customer image:Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon&gamma; expressing dog lymphocytes by flow cytometry.Image caption:Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, E
Primary Accession P30367
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names CC303
Calculated MW 15119 Da
Additional Information
Other Species Dog,Pig,Sh,Mustelid,Ga,Dolphin,Mink,Fin Whale,Hs
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 12 months from date of reconstitution.
Gene ID 280824
Other Names Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Lymphocyte stimulatory factor 1, IL4
Target/Specificity Mouse anti-Bovine Interleukin-4 antibody, clone CC303 recognizes bovine interleukin 4
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine Interleukin-4 Antibody, clone CC303 is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross react with cytokines from different animal species.
Vet. Immunol. Immunopathol. 88: 111 - 122. 2. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
Clin. Diagn. Lab. Immunol. 9: 1229 - 1234. 3. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virusinfections in a highly susceptible natural host.
J Gen Virol. 90: 2157-65. 4. Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.
J Comp Pathol. 143: 45-51. 5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. 6. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-relatedmolecules and cytokines in dogs.
Vet Immunol Immunopathol. 140: 175-80. 7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. 8. Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.
J Virol. 77: 7444-51. 9. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.
Can J Vet Res. 73: 137-43. 10. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
J Immunol Methods. 336: 71-7. 11. Wagner, B. et al. (2008) Characterization of monoclonal antibodies to equine interleukin-10 and detection of T regulatory 1 cells in horses.
Vet Immunol Immunopathol. 122: 57-64. 12. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
Int Arch Allergy Immunol. 144: 325-37. 13. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
BMC Vet Res. 11 (1): 92. 14. Dean, G.S. et al. (2005) Minimum infective dose of Mycobacterium bovis in cattle.
Infect Immun. 73 (10): 6467-71. 15. Araújo, M.S. et al. (2009) T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune.
Vaccine. 27 (7): 1008-17. 16. Yang, J. et al. (2012) Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo.
BMC Vet Res. 8: 25. 17. Taubert A et al. (2008) Antigen-induced cytokine production in lymphocytes of Eimeria bovis primary and challenge infected calves.
Vet Immunol Immunopathol. 126 (3-4): 309-20. 18. Geherin, S.A. et al. (2013) Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7.
Vet Immunol Immunopathol. 155 (1-2): 87-97. 19. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes
Veterinary Parasitology. 15 Feb [Epub ahead of print]

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