|Application ||FC, E|
|Calculated MW||15119 Da|
|Other Species||Dog,Pig,Sh,Mustelid,Ga,Dolphin,Mink,Fin Whale,Hs|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Lymphocyte stimulatory factor 1, IL4|
|Target/Specificity||Mouse anti-Bovine Interleukin-4 antibody, clone CC303 recognizes bovine interleukin 4|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine Interleukin-4 Antibody, clone CC303 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross react with cytokines from different animal species. Vet. Immunol. Immunopathol. 88: 111 - 122. 2. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 9: 1229 - 1234. 3. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virusinfections in a highly susceptible natural host. J Gen Virol. 90: 2157-65. 4. Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.J Comp Pathol. 143: 45-51. 5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15. 6. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-relatedmolecules and cytokines in dogs.Vet Immunol Immunopathol. 140: 175-80. 7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 8. Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.J Virol. 77: 7444-51. 9. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73: 137-43. 10. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.J Immunol Methods. 336: 71-7. 11. Wagner, B. et al. (2008) Characterization of monoclonal antibodies to equine interleukin-10 and detection of T regulatory 1 cells in horses.Vet Immunol Immunopathol. 122: 57-64. 12. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.Int Arch Allergy Immunol. 144: 325-37. 13. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92. 14. Dean, G.S. et al. (2005) Minimum infective dose of Mycobacterium bovis in cattle.Infect Immun. 73 (10): 6467-71. 15. Araújo, M.S. et al. (2009) T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune.Vaccine. 27 (7): 1008-17. 16. Yang, J. et al. (2012) Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo.BMC Vet Res. 8: 25. 17. Taubert A et al. (2008) Antigen-induced cytokine production in lymphocytes of Eimeria bovis primary and challenge infected calves.Vet Immunol Immunopathol. 126 (3-4): 309-20. 18. Geherin, S.A. et al. (2013) Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7.Vet Immunol Immunopathol. 155 (1-2): 87-97. 19. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytesVeterinary Parasitology. 15 Feb [Epub ahead of print]
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