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Anti-Human CD117 Antibody, clone 104D2

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD117 Antibody, clone 104D2  ABD12124
    Published customer image:Mouse anti Human CD117 antibody, clone 104D2 used for the evaluation of c-kit expression by flow cytometry.Image caption:Purity of Mast cells. A. Flow cytometry analysis of intracellular chymase expression. Human cord blood-derived mast cells labelled for intracellular mast-cell chymase expression (solid line), isotype control (Black area). Results shown are representative of four separate experiments. B. Toluidine blue staining of mast cells. Fluorescence microscopy of human cord blood-derived mast cells stained with Toluidine blue. Results shown are representative of at least three separate experiments. C. Flow cytometry analysis of cell surface expression of c-kit and FceRI. Human cord blood-derived mast cells labelled for cell-surface expression of c-kit and FceRI (left panel), and isotype controls (right panel). Results shown are representative of four separate experiments.From: Jayapal M, Tay HK, Reghunathan R, Zhi L, Chow KK, Rauff M, Melendez AJ.Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.BMC Genomics. 2006 Aug 16;7:210.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10721
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 104D2
Calculated MW 109865 Da
Additional Information
Other Species Cynomolgus Pr,B
Purification Purified IgG prepared by affinity chromatography
Immunogen MOLM-1 megakaryocyte cell line
Shelf Life 6 months from date of reconstitution.
Gene ID 3815
Other Names Mast/stem cell growth factor receptor Kit, SCFR,, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR
Target/Specificity Mouse anti-Human CD117 antibody, clone 104D2 recognizes the human CD117 cell surface antigen, a 145 kDa glycoprotein also known as c-kit. CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), some acute myeloid leukaemias and by mast cells.Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity.Mouse anti-Human CD117 antibody, clone 104D2 is non-neutralising.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD117 Antibody, clone 104D2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Broudy, V.C. et al. (1998) Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer.
Blood 91: 898-906. 2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp. Anim. 49: 97-110. 3. Jayapal, M. et al. (2006) Genome-wide gene expression profiling of human mast cells stimulated by IgE orFcepsilonRI-aggregation reveals a complex network of genes involved ininflammatory responses.
BMC Genomics. 7: 210 - 26 4. Randall, V.A. et al. (2008) Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles.
J Endocrinol. 197: 11-23.

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Cat# ABD12124
(40 western blots)
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