|Application ||WB, IHC-F, FC, E, IP|
|Calculated MW||25815 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||38C13, murine B cell line.|
|Shelf Life||18 months from date of despatch.|
|Other Names||CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, CD81, Cd81, Tapa1|
|Target/Specificity||Hamster anti-Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti-Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti-Ig and IL-4 induced proliferation (Maeckeret al.2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubensteinet al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD81 Antibody, clone Eat2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Clark, K. et al. (2001) PGRL is a major CD81 - associated protein on lymphocytes and distinguishes a new family of cell surface proteins. J. Immunol. 167: 5115 - 5121. 2. Maecker, H.T. et al. (2000) Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies.Hybridoma 19: 15-22. 3. Conde-Vancells, J. et al. (2010) Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.Proteomics Clin Appl. 4: 416-425. 4. Conde-Vancells, J. et al. (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.J Proteome Res. 7: 5157-66. 5. Takeda, Y. et al. (2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.J Biol Chem. 283: 26089-97. 6. Suzuki, M. et al. (2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.J Immunol. 182: 6485-93. 7. Ha, C.T. et al. (2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.J Leukoc Biol. 77: 948-57. 8. Pan, Q. et al. (2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).Gut. 61: 1330-9. 9. Jin, Y. et al. (2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.PLoS One. 8: e73706. 10. Royo, F. et al. (2013) Transcriptome of extracellular vesicles released by hepatocytes.PLoS One. 8: e68693. 11. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.Cancer Res. 61: 5248-54.
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