|Application ||WB, FC, IP|
|Calculated MW||42200 Da|
|Purification||Purified IgG prepared by ion exchange chromatography|
|Immunogen||CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, EMMPRIN, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF, CD147, BSG|
|Target/Specificity||Mouse anti-Human CD147 antibody, clone MEM-M6/1 recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain amd a more proximal Ig-like V-type domain in its extracellular region. Mouse anti-Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometric studies using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Kochet al.1999).Mouse anti-Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Kochet al.1999). In addition to clone MEM-M6/1 Bio-Rad AbD Serotec are also able to offer the MEM-M6/6 clone derived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human CD147 Antibody, clone MEM-M6/1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.Int Immunol. 11 (5): 777-86. 2. Yang, Y. et al. (2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis.Rheumatology (Oxford). 47: 1299-310. 3. Schneiderhan, W. et al. (2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay.J Cell Sci. 120: 512-9. 4. Chen, Y. et al. (2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.Cancer Lett. 278: 113-21. 5. Schneiderhan, W. et al. (2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro modelsGut. 58: 1391-8. 6. Wright, G.J. et al. (2014) Immunogenic compositions and expression systems.Patent Publication number. US20140093540 A1 7. Huang, Z. et al. (2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells.J Oral Pathol Med. 42: 541-6. 8. Hu, J. et al. (2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation.J Cell Mol Med. 14 (8): 2132-43. 9. Bernard, S.C. et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization.Nat Med. 20 (7): 725-31. 10. Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.Patent Publication number US20150110806 A1
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