|Application ||IHC-P, IHC-F|
|Calculated MW||122102 Da|
|Immunogen||Delipidated extract of human cream|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM|
|Target/Specificity||Mouse anti-Human Epithelial Membrane Antigen antibody, clone E29 recognizes epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD.This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Epithelial Membrane Antigen Antibody, clone E29 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Cordell, J. et al. (1985) Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.Br. J. Cancer. 52: 347-54. 2. Gusev, Y. et al. (2001) Rolling circle amplification: a new approach to increase sensitivity for immunohistochemistry and flow cytometry. Am. J. Pathol. 159 (1): 63 - 69. 3. ten Berge, R.L. (2001) MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.J Clin Pathol. 54: 933-9. 4. Cao, Y. et al. (1998) Immunohistochemical characterization of a panel of 56 antibodies with normal human small intestine, colon, and breast tissues.Tumour Biol. 19 Suppl 1: 88-99.
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