|Application ||FC, IP, Func|
|Calculated MW||84790 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine large granular lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18|
|Target/Specificity||Mouse anti-Pig CD18a, clone PNK-I recognizes porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmulleret al.1998). Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin β2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity (Dato and Kim 1990).CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules.CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor (Chenet al.2011).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD18a Antibody, clone PNK-I is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Dato, M.E. & Kim, Y.B. et al. (1990) Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells.J Immunol. 144:4452-4462. 2. Haverson, K. et al. (1999) T-cell populations in the pig intestinal lamina propria: memory cells with unusual phenotypic characteristics. Immunology 96:66-73. 3. Vanden Bergh, P.G. et al. (2009) Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.Vet Res. 40:1-10. 5. Chen, Z.W. et al. (2011) Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1ß, IL-8 and TNF-a in porcine alveolar macrophages.Vet Res. 42:25. 6. Vanden Bergh, P.G. et al. (2008) Probing of Actinobacillus pleuropneumoniae ApxIIIA toxin-dependent cytotoxicity towards mammalian peripheral blood mononucleated cells.BMC Res Notes 1:121. 7. Ebdrup, L. et al. (2008) Dynamic expression of the signal regulatory protein alpha and CD18 on porcine PBMC during acute endotoxaemia.Scand J Immunol. 68:430-7.8. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39:54.
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