|Application ||WB, FC, IP|
|Calculated MW||32995 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Other Names||Leukocyte surface antigen CD47, Integrin-associated protein, IAP, CD47, Cd47|
|Target/Specificity||Mouse anti-Rat CD47 antibody, clone OX-101 recognizes the rat homologue of human CD47, also known as integrin associated protein (IAP). Rat CD47 is a 303 amino acid ӯ33 kDa multi pass transmembrane glycoprotein bearing a single V-type Ig-like domain and has been identified as the ligand for the rat signal regulatory protein, SIRP (Vernon-Wilsonet al.2000). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.Mouse anti-Rat CD47 antibody, clone OX-101 has been reported to block the interaction of CD47 and SIRP (Vernon-Wilsonet al.2000).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat CD47 Antibody, clone OX-101 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Vernon-Wilson, E. F. et al. (2000) CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha1. Eur. J. Immunol. 30: 2130-2137. 2. de Vries, H.E. et al. (2002) Signal regulatory protein alpha - CD47 interactions are required for the transmigration of monocytes across cerebral endothelium. J. Immunol. 168: 5832 - 5839. 3. Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells. Cell Mol Life Sci. 66: 1271-82. 4. Barrès, C. et al. (2010) Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages.Blood. 115: 696-705. 5. Kurihara H et al. (2010) SIRP-alpha-CD47 system functions as an intercellular signal in the renal glomerulus.Am J Physiol Renal Physiol. 299 (3): F517-27.
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