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Anti-Bovine CD172a Antibody, clone CC149 (RPE-CY5)

Mouse Anti-Bovine Monoclonal Antibody

     
  • IF - Anti-Bovine CD172a Antibody, clone CC149 (RPE-CY5) ABD12147
    Published customer image:Mouse anti Bovine CD172α antibody, clone CC149 used for the detection of SIRP1α expressing cells in cultured bovine PBMCs by immunofluorescence.Image caption:CD172a cells bind rESAT-6:CFP-10 and M. bovis BCG. CD172a+ cells (PE-labeled) were isolated by high-speed cell sorting (>98% purity) from rESAT-6:CFP-10-stimulated PBMC 6d cultures; incubated with rESAT-6:CFP-10-FITC or BCG-FITC for 2–96 hrs; and evaluated by fluorescence microscopy. (A) rESAT-6:CFP-10-FITC bound to the surface of CD172a+ cells in a focal pattern (24 hr cultures shown). Labeling patterns were similar at 2, 24 (shown in Fig. 3A) and 96 hrs after addition of rESAT-6:CFP-10 (FITC) to cells, except for increased polarization of staining at 96 hrs. (B) Over the 96 hr culture period, PE-staining (red) used for CD172a+ cell sorting persisted (24 hr cultures shown) with a polar distribution, possibly due to capping of antibody bound to CD172a. However, rESAT-6:CFP-10-labeling (green) did not overlap with CD172a-PE labeling (red). (C) M. bovis BCG was detected in association with CD172a+ cells at 2 (not shown) and 24 hrs (green, intact bacteria associated with the cell surface, panel C) after addition of the live bacteria to the CD172a+ cell culture and by 96 hrs, M. bovis BCG was internalized and mostly degraded (D). White bar = 10 µm.From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. Signal Regulatory Protein a (SIRPa)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.PLoS ONE 4: e6414.
  • FC - Anti-Bovine CD172a Antibody, clone CC149 (RPE-CY5) ABD12147
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD172a followed by Goat anti Mouse IgG:FITC
  • FC - Anti-Bovine CD172a Antibody, clone CC149 (RPE-CY5) ABD12147
    Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 used for the identification of CD172a expressing cells in bovine blood by flowcytometry.Image caption:In vitro characterization of the dual immunosuppressive effect of tick saliva on inflammatory-induced mobilization of monocyte-derived mononuclear phagocytes. A-E Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells for 48 h according to the reverse-transmigration protocol. Left contour plots show conditions with zymosan alone whereas right contour plots show conditions with zymosan and tick saliva. B Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with zymosan alone or with zymosan and saliva. C Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. D Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. E Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. B-E The value for the control condition (zymosan alone) was arbitrarily set to 1. Data are presented as the mean?±?SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P?
  • FC - Anti-Bovine CD172a Antibody, clone CC149 (RPE-CY5) ABD12147
    Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD172a:RPE-Cy5
  • FC - Anti-Bovine CD172a Antibody, clone CC149 (RPE-CY5) ABD12147
    Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 used for the identification of CD172a expressing cells in bovine blood by flowcytometry.Image caption:Adaptation of the reverse-transmigration in vitro model with bovine cells to recapitulate monocyte-derived cells mobilization from blood to the draining lymph node under inflammatory condition. A-D Schematic representation of the in vitro differentiation of blood monocytes into macrophages and Mo-DCs using the reverse-transmigration model. E-I Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells according to the reverse-transmigration protocol. Non-adherent and low-adherent cells within the top and bottom sections of the transwell were processed for cytometry analysis. Left contour plots show the conditions in the absence of any exogenous stimulus whereas right contour plots show conditions with zymosan. Cells were labeled with anti-SIRP1-α to discriminate monocyte-derived cells from endothelial cells. The expression of MHCII within the SIRP1-α+ population was also determined to discriminate within monocyte-derived cells potential macrophages (MHCII+) and potential Mo-DC (E). The percentages of SIRP1-α+MHCII+, SIRP1-α+MHCIIhi and BAECs in each representative dot plot are indicated (E). F Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with or without zymosan stimulation. G Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. H Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with or without zymosan stimulation. i Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. F-I The value of the control condition was arbitrarily set to 1. Data are presented as the mean?±?SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P?
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession O46631
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names CC149
Calculated MW 55093 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 12 months from date of reconstitution.
Gene ID 327666
Other Names Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, CD172a, SIRPA, MYD1, PTPNS1, SHPS1, SIRP
Target/Specificity Mouse anti-Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA. CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47.Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin.
Preservative & Stabilisers 0.09% Sodium Azide; Stabilizing agent
Storage Storage at +4ºC. DO NOT FREEZE.
PrecautionsAnti-Bovine CD172a Antibody, clone CC149 (RPE-CY5) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Howard, C.J. et al. (1999) Dendritic cells in cattle: phenotype and function.
Vet Immunol Immunopathol. 72 (1-2): 119-24. 2. Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
Immunology. 126:201-8 3. Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.
PLoS One. 4: e6414. 4. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitroandin vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. 5. Smith, R. et al. (2003) A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes.
Blood.102:2532-40. 6. Jensen, K. et al. (2014) Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation.
Vet Immunol Immunopathol. 158 : 224-32. 7. Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9. 8. Hussen J et al. (2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
Dev Comp Immunol. 47 (2): 169-77. 9. Eger, M. et al. (2015) Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets.
Vet Immunol Immunopathol. 166 (1-2): 33-42. 10. Vachiery N et al. (2015) An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 46 (1): 117.

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