|Application ||IHC-F, IF, FC, E|
|Calculated MW||38224 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Recombinant human HLA-G refolded with beta 2 microglobulin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, MHC class I antigen G, HLA-G, HLA-6.0, HLAG|
|Target/Specificity||Mouse anti-Human HLA G antibody, clone MEM-G/9 recognizes human HLA-G, a non-classical major histocompatibility complex (MHC) molecule. HLA-G expression is restricted to trophoblast cells and some medullary thymic epithelial cells. Several isoforms of the HLA-G molecule exist, which include the membrane bound isoforms HLA-G1 – G4 and soluble isoforms HLA-G5 – G7. Clone MEM-G/9 specifically recognizes surface expressed native HLA-G1, when associated with beta 2 microglobulin, but not does recognize the isoforms HLA-G2, G3 and G4. CMouse anti-Human HLA G antibody, clone MEM-G/9 has also been reported to recognize the soluble isoform HLA-G5.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human HLA G Antibody, clone MEM-G/9 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.Tissue Antigens. 55 (6): 510-8. 2. Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.Hum Immunol. 64 (3): 315-26. 3. Kotze, D.J. et al. (2010) Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome.J Assist Reprod Genet. 27 (6): 309-16. 4. Guetta, E. et al (2005) Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.J Histochem Cytochem. 53: 337-9. 5. Hiby, S.E. et al (2010) Maternal activating KIRs protect against human reproductive failure mediated by fetal HLA-C2J Clin Invest. 120: 4102-10. 6. Sher, G. et al. (2005) Influence of early ICSI-derived embryo sHLA-G expression on pregnancy and implantation rates: a prospective study.Hum Reprod. 20: 1359-63. 7. Sher, G. et al. (2005) Soluble human leukocyte antigen G expression in phase I culture media at 46 hours after fertilization predicts pregnancy and implantation from day 3 embryo transfer.Fertil Steril. 83: 1410-3. 8. Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.Mol Hum Reprod. 17: 577-86. 9. Manaster, I. et al. (2012) MiRNA-mediated control of HLA-G expression and function.PLoS One. 7: e33395. 10. Nückel, H. et al. (2005) HLA-G expression is associated with an unfavorable outcome and immunodeficiency in chronic lymphocytic leukemia.Blood. 105: 1694-8. 11. Yao, Y.Q. et al. (2005) Differential expression of alternatively spliced transcripts of HLA-G in human preimplantation embryos and inner cell masses.J Immunol. 175 (12): 8379-85. 12. de Carvalho, J.F. et al. (2012) Heparin increases HLA-G levels in primary antiphospholipid syndrome.Clin Dev Immunol. 2012: 232390. 13. Guenther, S. et al. (2012) Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis.Int J Mol Sci. 13 (7): 9069-80. 14. Apps, R. et al. (2011) Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.Placenta. 32 (1): 33-43. 15. Lim DS et al. (2014) The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.J Immunol. 193 (10): 5065-75.
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