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Anti-Human CD56 Antibody, clone MEM-188

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD56 Antibody, clone MEM-188  ABD12150
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD56
  • FC - Anti-Human CD56 Antibody, clone MEM-188  ABD12150
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD56:Biotin
  • FC - Anti-Human CD56 Antibody, clone MEM-188  ABD12150
    Published customer image:FITC conjugated Mouse anti Human CD56 antibody, clone MEM-188 used for the identificationof CD56 positive cells from human peripheral blood mononuclear cell populations by flow cytometry.Image caption: Intracellular GM-CSF staining on CD56+ cells from hPBMC. (A) In the presence of the indicated ODN plus IL2: GM-CSF content in the cell culture without ODN stimulation was used as a control. Data are shown as mean +SD and are representative of three similar experiments. A specific response was defined as a response where the percentage of GM-CSF in CD56+ lymphocytes was at least 2-fold below the background observed in the medium control. The asterisk indicates statistically significant differences (p
  • FC - Anti-Human CD56 Antibody, clone MEM-188  ABD12150
    Published customer image:AlexaFluor-647 conjugated Mouse anti Human CD56 antibody used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.Image caption:HLA-DR+ Accessory Cells Provide Activating Signals to NK Cells PBMCs from malaria-naïve donors were depleted of different populations of accessory cells, and NK cell responses to iRBCs were analysed after 24 h of co-culture. (A) Representative example of NK cell IFN-? responses in undepleted PBMCs (top left), PBMCs depleted of all HLA-DR+ antigen-presenting cells (top right), CD14+ monocyte–depleted PBMCs (middle left), CD19+ B cell–depleted PBMCs (middle right), CD1c+ mDC–depleted PBMCs (bottom left), and BDCA-4+ pDC–depleted PBMCs (bottom right). FACS plots are gated on CD3- CD56+ lymphocytes. Percentages indicate the proportion of CD3- CD56+ NK cells that were positive for IFN-?; data are based on the collection of 100,000 total events. (B) NK cell IFN-? production after depletion of different antigen-presenting cell populations. Percentage of IFN-? NK cells relative to the response in undepleted PBMCs is shown; each spot represents data for a different donor. *, p < 0.05; ***, p < 0.0001; p–values are for paired t tests of the difference between depleted and undepleted cells:From: Newman KC, Korbel DS, Hafalla JC, Riley EM Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-? Responses to Malaria. PLoS Pathog 2: e118.
  • FC - Anti-Human CD56 Antibody, clone MEM-188  ABD12150
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD56:Alexa Fluor® 647
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13591
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names MEM-188
Calculated MW 94574 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Gene ID 4684
Other Names Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56, NCAM1, NCAM
Target/Specificity Mouse anti-Human CD56 antibody, clone MEM-188 recognizes CD56 a 140kDa cell surface glycoprotein expressed primarily by natural killer cells and a subset of T cells in the peripheral blood.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD56 Antibody, clone MEM-188 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Costa, al (1997) CD56 Workshop Panel Report.
In: Leucocyte typing VI. White cell differentiation antigens. Garland Publishing Inc. New York and London. 271-2. 2. Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J. Immunol. 166: 2364 - 2371. 3. Staffler, G. et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
J. Immunol. 171: 1707 - 1714. 4. Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
Cell Stem Cell. 3: 301-13. 5. Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118. 6. Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
Mol Hum Reprod. 17: 577-86. 7. Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
Hepatology. 51: 2030-9. 8. Rodriguez JM et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells.
PLoS One. 10 (2): e0117484.

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Cat# ABD12150
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