|Application ||IHC-F, IF, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of 12kD and 46kD. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine CD1w2 Antibody, clone CC20 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells. Vet. Immunol. Immunopathol. 60: 15-31. 2. Hein, W .R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep. Vet. Immunol. Immunopathol. 27: 28-30. 3. Howard, C. J. et al. (1993) General summary of workshop findings for cattle. Vet. Immunol. Immunopathol. 39: 25 - 48. 4. Howard, C. J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues. Vet. Immunol. Immunopathol. 39: 77 - 83. 5. Rhind, S. M. (2001) CD1--the pathology perspective.Vet Pathol. 38(6): 611-619. 6. Howard, C. J. et al. (1993) Summary of workshop findings for cattle. Vet. Immunol. Immunopathol. 39: 25 - 47. 7. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32: 837-49. 8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.Immunology. 107: 366-72. 9. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.Vet Immunol Immunopathol. 129: 82-92. 10. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.J Histochem Cytochem. 54: 1021-30. 11. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.Vet Immunol Immunopathol. 131: 1-8. 12. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.Vet Pathol. 39: 74-83. 13. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.Am J Pathol. 148: 1699-708. 14. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.Vet Immunol Immunopathol. 96: 19-30. 15. Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.Transbound Emerg Dis. Aug 25. [Epub ahead of print]
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