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>   home   >   Products   >   Primary Antibodies   >   Anti-BrdU Antibody, clone BU1/75 (ICR1) (FITC)   

Anti-BrdU Antibody, clone BU1/75 (ICR1)

     
  • IHC - Anti-BrdU Antibody, clone BU1/75 (ICR1)  ABD12156
    Published customer image:Rat anti BrdU antibody used for the identification of proliferating cells in the axolotl brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Ventricular zones with variable proliferative activity can be detected throughout the axolotl central nervous system. (A) Structure of the axolotl brain showing the levels at which sections ventricular zone proliferation was analyzed. B = anterior telencephalon and olfactory nerve, C = anterior telencephalon and olfactory bulb, D = posterior telencephalon, E = mesencephalon, F = cerebellum, G = rhombencephalon; on: olfactory nerve. Scale bar = 1?mm. (B – G) Cumulative BrdU+ cell numbers on five sections from each level depicted in A on which individual labeled cells are marked with a red dot. Regions of the brain are marked.; on: olfactory nerve; ob: olfactory bulb; cp: choroid plexus.From: Maden M, Manwell LA, Ormerod BK. Proliferation zones in the axolotl brain and regeneration of the telencephalon.Neural Dev. 2013 Jan 17;8:1.
  • IHC-P - Anti-BrdU Antibody, clone BU1/75 (ICR1)  ABD12156
    Published customer image:Rat anti BrdU antibody, clone Bu1/75 used for the detection of BrdU incorporating cells in the intestine of rats by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:BrdU labeling of the small intestine after i.v. administration. BrdU (50 mg/kg b.wt.), a thymidine analogue, was given 2 h prior to removing the intestine. The dark labeling by BrdU is exclusively located to the nuclei of intestinal crypt cells. Mayer's haematoxylin.From: Lundgren O, Jodal M, Jansson M, Ryberg AT, Svensson L Intestinal Epithelial Stem/Progenitor Cells Are Controlled by Mucosal Afferent Nerves.PLoS ONE 6(2): e16295.
  • IHC-P - Anti-BrdU Antibody, clone BU1/75 (ICR1)  ABD12156
    Published customer image:Rat anti BrdU antibody, clone Bu1/75 used for the detection of proliferating cells in the rat kidney by immunofluorescence on formalin fixed, paraffing embedded tissue sections.Image caption:Phenotypes of BrdU-positive tubular cells in the UUO kidneys. a Expression of myofibroblast-related markers in kidneys at 7 days after UUO. BrdU , markers , and DAPI . Magnification, ×1000. Arrowheads indicate BrdU-positive cells coexpressing myofibroblast markers. b Number of alpha-SMA-positive cells and alpha-SMA/BrdU double-positive cells in the interstitium of contralateral and UUO kidneys at 8 days after operation. Values are means?±?SE (n?=?5). c Expression of AQP-1 in kidneys at 7 days after UUO. Arrowheads indicate BrdU-positive cells losing AQP-1 expression. BrdU , AQP-1 , and DAPI . Magnification, ×1000. d Expression of inflammatory cell markers in kidneys at 7 days after UUO. DAPI . Magnification, ×1000.From: Nakasatomi M, Maeshima A, Mishima K, Ikeuchi H, Sakairi T, Kaneko Y, Hiromura K, Nojima Y. Novel approach for the detection of tubular cell migration into the interstitium during renal fibrosis in rats.Fibrogenesis Tissue Repair. 2015 Jul 10;8:12.
  • IHC-P - Anti-BrdU Antibody, clone BU1/75 (ICR1)  ABD12156
    Published customer image:Rat anti BrdU antibody, clone Bu1/75 used for the detection of proliferating cells in the rat kidney by immunofluorescence on formalin fixed, paraffing embedded tissue sections.Image caption:Localization of BrdU-positive cells in the normal rat kidneys after long-term BrdU labeling. a–c BrdU was given intraperitoneally to normal rats for 4 weeks. Double staining of BrdU with several tubular (a), glomerular (b), and interstitial (c) markers was performed. Markers: aquaporin-1 (AQP-1), lotus tetragonolobus agglutinin , Tamm-Horsfall glycoprotein , aquaporin-2 (AQP-2), alpha-SMA, vimentin, CD31, WT1, and aminopeptidase P. DAPI . Magnification, ×1000. d Quantitative analysis of percentage of BrdU-positive cells per total marker-positive cells. Values are means?±?SE (n?=?5).From: Nakasatomi M, Maeshima A, Mishima K, Ikeuchi H, Sakairi T, Kaneko Y, Hiromura K, Nojima Y. Novel approach for the detection of tubular cell migration into the interstitium during renal fibrosis in rats.Fibrogenesis Tissue Repair. 2015 Jul 10;8:12.
  • FC - Anti-BrdU Antibody, clone BU1/75 (ICR1)  ABD12156
    HL-60 cells were pulse labeled with BrdU for 45 minutes prior to harvesting and then incubated with primary antibody Rat anti BrdU clone BU1/75 diluted 1/100 followed by Rabbit anti Rat IgG secondary antibody FITC-conjugated, diluted 1/200
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, FC
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names BU1/75 (ICR1)
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti-BrdU antibody clone BU1/75 (ICR1), recognizes bromodeoxyuridine (known as BrdU or BrdUrd). Rat anti-BrdU antibody clone BU1/75 (ICR1) reacts with BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU.Rat anti-BrdU antibody, clone BU1/75 (ICR1) cross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Atenet al.1992). BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivoandin vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqziet al.2004) or in drinking water (Moseret al.2004)Clone BU1/75 (ICR1) has been used to detect CldU to study the speed of DNA replication fork (Bugleret al.2010) , in the detection of CldU label retaining stem cells (Kimotoet al.2008) and label retaining neurons (Murataet al.2011).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-BrdU Antibody, clone BU1/75 (ICR1) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

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PLoS One. 6: e16295. 22. Knopf, F. et al. (2011) Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin.
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Mol Cancer Res. 11 (4): 370-80. 29. Maden, M. et al. (2013) Proliferation zones in the axolotl brain and regeneration of the telencephalon.
Neural Dev. 8 (1): 1. 30. Wu X et al. (2015) Vasoactive intestinal polypeptide promotes intestinal barrier homeostasis and protection against colitis in mice.
PLoS One. 10 (5): e0125225. 31. Nakasatomi, M. et al. (2015) Novel approach for the detection of tubular cell migration into the interstitium during renal fibrosis in rats.
Fibrogenesis Tissue Repair. 8: 12. 32. Hirota K et al. (2015) The POLD3 subunit of DNA polymerase δ can promote translesion synthesis independently of DNA polymerase ζ.
Nucleic Acids Res. 43 (3): 1671-83. 33. Nouri N et al. (2015) Excessive Wnt/beta-catenin signaling promotes midbrain floor plate neurogenesis, but results in vacillating dopamine progenitors.
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