|Application ||IHC-F, FC, IP|
|Calculated MW||40922 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.|
|Immunogen||Papain solubilised HLA-A2|
|Shelf Life||18 months from date of despatch.|
|Other Names||HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A*2, HLA-A, HLAA|
|Target/Specificity||Mouse anti-Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti-Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti-Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity..|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human HLA A2 Antibody, clone BB7.2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Parham, P. & Brodsky, F.M. (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.Hum Immunol. 3 (4): 277-99. 2. Hogan, K.T. & Brown, S.L. (1992) Localization and characterization of serologic epitopes on HLA-A2.Hum Immunol. 33 (3): 185-92. 3. Harig, S. et al. (2001) Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukocyte antigen-A2 binding residues.Blood. 98 (10): 2999-3005. 4. Pantenburg, B. et al. (2010) Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells. Am J Trop Med Hyg. 82:600-7. 5. Duncan, L.M. et al. (2010) Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression.J Immunol. 184: 6978-85. 6. Wang, B. et al. (2004) Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.Blood. 104: 200-6. 7. Wooldridge, L. et al. (2007) Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region.Eur J Immunol. 37: 1323-33. 8. Wooldridge, L. et al. (2010) MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs.J Immunol. 184: 3357-66. 9. Popovic, J. et al. (2011) The only proposed T-cell epitope derived from the TEL-AML1 translocation is not naturally processed.Blood. 118 (4): 946-54. 10. Schmidt, J. et al. (2011) Reversible Major Histocompatibility Complex I-Peptide Multimers Containing Ni2+-Nitrilotriacetic Acid Peptides and Histidine Tags Improve Analysis and Sorting of CD8+ T Cells.J Biol Chem. 286: 41723-35.
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