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Anti-Human BLTR Antibody, clone 202/7B1

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human BLTR Antibody, clone 202/7B1  ABD12165
    Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:Alexa Fluor® 647
  • FC - Anti-Human BLTR Antibody, clone 202/7B1  ABD12165
    Staining of human peripheral blood monocytes with Mouse anti Human BLTR
  • IF - Anti-Human BLTR Antibody, clone 202/7B1  ABD12165
    Published customer image:Mouse anti Human BLTR antibody, clone 202/7B1 used for the detection of Leukotriene B4 receptor 1 expressing cultured peripheral blood mononuclear cells by immunofluorescence.Image caption:Leukotriene B4 biosynthetic enzymes and receptors are expressed in both macrophages and osteoclasts. (a) Quantification of leukotriene B4 levels by enzyme-linked immunoassay in the conditioned medium of human peripheral blood mononuclear cells treated with 25 ng/ml macrophage colony-stimulating factor (M-CSF) and 10 ng/ml interleukin 23 for 3 days. Representative data from three experiments are shown. **P < 0.01. (b) Immunofluorescence of human PBMCs cultured with either M-CSF alone for 8 days or M-CSF + receptor activator of nuclear factor ?B ligand for 14 days showing the expression of BLT1, BLT2, 5-lipoxygenase (5-LO) and leukotriene A4 hydrolase in mononuclear and multinucleated giant cells. Representative images from three experiments are shown. 4',6-diamidino-2-phenylindole is blue and BLT1, BLT2, 5-LO and LTA4H are in red. The scale bars represent 20 µm in Figure 1b.From:Dixit N, Wu DJ, Belgacem YH, Borodinsky LN, Gershwin M, Adamopoulos IE. Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis. Arthritis Res Ther. 2014 Dec 2;16(6):496.
  • FC - Anti-Human BLTR Antibody, clone 202/7B1  ABD12165
    Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:FITC
  • FC - Anti-Human BLTR Antibody, clone 202/7B1  ABD12165
    Staining of human peripheral blood monocytes with Mouse anti Human BLTR: Azide Free
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15722
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 202/7B1
Calculated MW 37557 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen HeLa cell transfectants expressing human BLTR.
Shelf Life 18 months from date of despatch.
Gene ID 1241
Other Names Leukotriene B4 receptor 1, LTB4-R 1, LTB4-R1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, P2Y purinoceptor 7, P2Y7, LTB4R, BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7
Target/Specificity Mouse anti-Human BLTR antibody, clone 202/7B1 recognizes the human BLTR molecule, the leukotriene B4 receptor.BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human BLTR Antibody, clone 202/7B1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Pettersson, A. et al. (2000) First generation monoclonal antibodies identifying the human leukotriene B receptor-1.
Biochem. Biophys. Res. Commun. 279: 520 - 525. 2. Costa, M.F. et al. (2010) Leukotriene B4 mediates gammadelta T lymphocyte migration in response to diverse stimuli.
J Leukoc Biol. 87: 323-32. 3. Islam, S.A. et al. (2006) The leukotriene B4 lipid chemoattractant receptor BLT1 defines antigen-primed T cells in humans.
Blood. 107: 444-53. 4. Medoff, B.D. et al. (2005) BLT1-mediated T cell trafficking is critical for rejection and obliterative bronchiolitis after lung transplantation.
J Exp Med. 202: 97-110. 5. Serezani, C.H. et al. (2009) FcgammaRI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions.
Blood. 114: 3316-24. 6. Heller, E.A. et al. (2005) Inhibition of atherogenesis in BLT1-deficient mice reveals a role for LTB4 and BLT1 in smooth muscle cell recruitment.
Circulation. 112: 578-86. 7. Thomas, S.Y. et al. (2007) Multiple chemokine receptors, including CCR6 and CXCR3, regulate antigen-induced T cell homing to the human asthmatic airway.
J Immunol. 179: 1901-12. 8. Sabirsh, A. et al. (2005) Fluorescent leukotriene B4: potential applications.
J Lipid Res. 46: 1339-46. 9. Loell, I. et al. (2012) Activated LTB4 pathway in muscle tissue of patients with polymyositis or dermatomyositis.
Ann Rheum Dis. 72: 293-9. 10. Wang, L. et al. (2012) BLT1-Dependent Alveolar Recruitment of CD4+CD25+ Foxp3+ Regulatory T Cells is Important for Resolution of Acute Lung Injury.
Am J Respir Crit Care Med.186: 989-98. 11. Laarman, A.J. et al.(2012)Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
EMBO J. 31: 3607-19. 12. Dixit, N. et al. (2014) Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis.
Arthritis Res Ther. 16: 496.

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Cat# ABD12165
(40 western blots)
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