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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Bovine Interleukin-10 Antibody, clone CC320    

Anti-Bovine Interleukin-10 Antibody, clone CC320 (Biotin)

Mouse Anti-Bovine Monoclonal Antibody

  • E - Anti-Bovine Interleukin-10 Antibody, clone CC320 (Biotin) ABD12166
    Published customer image:Biotinylated Mouse anti Bovine interleukin-10 antibody, clone CC320 used as a detection reagent for the determination of IL-10 levels in ovine plasma by ELISAImage caption:Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p = 0.0001.From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.PLoS ONE 10: e0124144.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P43480
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC320
Calculated MW 20438 Da
Additional Information
Other Species Hs,Sh,Ga
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Plasmid cDNA encoding bovine IL-10
Shelf Life 18 months from date of despatch.
Gene ID 281246
Other Names Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Target/Specificity Mouse anti-Bovine Interleukin-10 antibody, clone CC320 recognizes bovine IL-10.Mouse anti-Bovine Interleukin-10 antibody, clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buzaet al.2004).
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine Interleukin-10 Antibody, clone CC320 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
Vet Immunol Immunopathol. 85 (3-4): 213-23. 2. Buza JJ et al. (2004) Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium aviumsubsp.paratuberculosis in experimentally infected cattle with paratuberculosis.
Infect Immun. 72 (4): 2425-8. 3. Scandurra, G.M. et al. (2010) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
Infect Immun. 78 (3): 1383-9. 4. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.avium.
J Leukoc Biol. 83 (1): 48-55. 5. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
Int Arch Allergy Immunol. 144: 325-37. 6. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. 7. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. colimastitis.
Funct Integr Genomics. 10: 21-38. 8. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
Clin Experiment Ophthalmol. 38: 405-13. 9. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. 10. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium aviumsubsp.paratuberculosis-infected goats.
Clin Vaccine Immunol. 16 (7): 1003-11. 11. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. 12. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
J Virol. 82: 1777-86. 13. Ma&347;lanka T et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
Pol J Vet Sci. 15 (1): 11-20. 14. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium aviumsubsp.paratuberculosis.
J Comp Pathol. 148: 157-72. 15. Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
PLoS One. 10 (5): e0124144.

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Cat# ABD12166
(40 western blots)
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