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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD229 Antibody, clone HLy9.1.25    

Anti-Human CD229 Antibody, clone HLy9.1.25

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD229 Antibody, clone HLy9.1.25  ABD12170
    Published customer image:Mouse anti Human CD229 antibody, clone HLy9.1.25 used for the detection of SLAMF3 using flow cytometry.Image caption:Expression of SLAMF3 assessed by flow cytometry analysis after transfection with vector coding for SLAMF3 or an empty vector (Mock) in Huh-7 and HepG2 cells. SLAMF3 staining (in grey) is overlaid by negative control (in white). One of four independent experiments is shown. doi:10.1371/journal.pone.0082918.s002From: Marcq I, Nyga R, Cartier F, Amrathlal RS, Ossart C, et al. Identification of SLAMF3 as an Inhibitor of Hepatocellular Carcinoma Cell Proliferation and Tumour Progression. PLoS ONE 8: e82918.
  • FC - Anti-Human CD229 Antibody, clone HLy9.1.25  ABD12170
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD299: Azide Free
  • IF - Anti-Human CD229 Antibody, clone HLy9.1.25  ABD12170
    Published customer image:Mouse anti Human CD229 antibody, clone HLy9.1.25 used for the detection of SLAMF3 by immunofluorescence.Image caption:Effect of SLAMF3 expression on the organization of the actin cytoskeleton. Cells (Huh-7) were stained with phalloidin (rhodamine, red) and anti-SLAMF3 (FITC, green) and SLAMF3 positive (Huh-7-SLAMF3pos) and SLAMF3-negative (Huh-7-SLAMF3pos) cells were examined under the microscope. One representative of two independent experiments is shown.From: Marcq I, Nyga R, Cartier F, Amrathlal RS, Ossart C, et al. Identification of SLAMF3 as an Inhibitor of Hepatocellular Carcinoma Cell Proliferation and Tumour Progression. PLoS ONE 8: e82918.
  • FC - Anti-Human CD229 Antibody, clone HLy9.1.25  ABD12170
    Published customer image:Mouse anti Human CD229 antibody, clone HLy9.1.25 used for the detection of SLAMF3 using flow cytometry.Image caption:SLAMF3 is expressed by hepatocytes. (A) SLAMF3 expression was analysed by flow cytometry analysis in HHPHs and in Huh-7, HepG2 and Hep3B human HCC cell lines. SLAMF3 expression in Jurkat lymphocytes was used as a positive control; SLAMF3 staining (in grey) is overlaid by the negative control (in white) and corresponds to one representative of five independent experiments. (B) Western blot analysis of proteins extracted from Huh-7 cells, HepG2 cells or HHPHs, with a mAb against SLAMF3's first extracellular domain or an anti-actin antibody as a control. One of four independent experiments is presented here. (C) Expression of SLAMF3 transcripts in hepatocytes. After reverse transcription, SLAMF3 cDNA was amplified by PCR using specific primers. GAPDH was amplified as a control gene and pure H2O was used as a PCR control. The Daudi and Jurkat human lymphocyte cell lines were used as positive controls and the monkey kidney COS-7 cell line was used as a negative control. One of three independent experiments is shown here; (D) SLAMF3 mRNA was assayed by Q-PCR in (i) HHPHs, Huh-7 cells and HepG2 cells, Daudi B lymphocytes and Jurkat T lymphocytes (positive controls) and the green monkey kidney COS-7 cell line (a negative control). Results are presented as the mean ± SD (n = 6) ***p
  • FC - Anti-Human CD229 Antibody, clone HLy9.1.25  ABD12170
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD229 : RPE
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IP
Primary Accession Q9HBG7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names HLy9.1.25
Calculated MW 72139 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Mouse pre-B cell line 300.19 transfected with CD229 cDNA.
Shelf Life 18 months from date of despatch.
Gene ID 4063
Other Names T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229, LY9
Target/Specificity Mouse anti-Human CD229 antibody, clone HLy9.1.25 recognizes the human cell surface antigen CD229 also known as T-lymphocyte surface antigen Ly-9 or SLAM family member 3. CD229 is a 608 amino acid single pass type I transmembrane glycoprotein of ~120 kDa as evaluated by immunoprecipitation of cells transfected with the full length human CD229 cDNA. However immunoprecipitation of CD229 from Daudi cell lysates with dlone HLy9.1.25 yields bands of 120 kDa corresponding to the full length CD229 and a 100 kDa band attributed to an alternatively spliced isoform lacking the fourth Ig-like domain (de la Fuente et al.2001).Human CD299 is expressed on thymocytes, T-cells and B-cells (Del Valle et al.2003). CD229 has also been described as a tumor associated antigen in chronic lymphocytic leukemia (Bundet al.2006) and has been implicated in the development of spontaneous autoantiboy production to nuclear antigens in mice and is potentially a target for the treatment of autoimmunity (de Salort et al.2013).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD229 Antibody, clone HLy9.1.25 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. de la Fuente, M.A. et al. (2001) Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9.
Blood. 97 (11): 3513-20. 2. Sayós J et al. (2001) Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP.
Blood. 97 (12): 3867-74. 3. Cartier, F. et al. (2014) The expression of the hepatocyte SLAMF3 (CD229) receptor enhances the hepatitis C virus infection.
PLoS One. 9: e99601. 4. Marcq, I. et al. (2013) Identification of SLAMF3 (CD229) as an Inhibitor of Hepatocellular Carcinoma Cell Proliferation and Tumour Progression
PLoS One. 8: e82918. 5. Margraf, S. et al. (2015) A polymorphism in a phosphotyrosine signalling motif of CD229 (Ly9, SLAMF3) alters SH2 domain binding and T-cell activation.
Immunology. 146 (3): 392-400.

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