|Application ||FC, IP|
|Calculated MW||72139 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Mouse pre-B cell line 300.19 transfected with CD229 cDNA.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229, LY9|
|Target/Specificity||Mouse anti-Human CD229 antibody, clone HLy9.1.25 recognizes the human cell surface antigen CD229 also known as T-lymphocyte surface antigen Ly-9 or SLAM family member 3. CD229 is a 608 amino acid single pass type I transmembrane glycoprotein of ~120 kDa as evaluated by immunoprecipitation of cells transfected with the full length human CD229 cDNA. However immunoprecipitation of CD229 from Daudi cell lysates with dlone HLy9.1.25 yields bands of 120 kDa corresponding to the full length CD229 and a 100 kDa band attributed to an alternatively spliced isoform lacking the fourth Ig-like domain (de la Fuente et al.2001).Human CD299 is expressed on thymocytes, T-cells and B-cells (Del Valle et al.2003). CD229 has also been described as a tumor associated antigen in chronic lymphocytic leukemia (Bundet al.2006) and has been implicated in the development of spontaneous autoantiboy production to nuclear antigens in mice and is potentially a target for the treatment of autoimmunity (de Salort et al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD229 Antibody, clone HLy9.1.25 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. de la Fuente, M.A. et al. (2001) Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9.Blood. 97 (11): 3513-20. 2. Sayós J et al. (2001) Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP.Blood. 97 (12): 3867-74. 3. Cartier, F. et al. (2014) The expression of the hepatocyte SLAMF3 (CD229) receptor enhances the hepatitis C virus infection.PLoS One. 9: e99601. 4. Marcq, I. et al. (2013) Identification of SLAMF3 (CD229) as an Inhibitor of Hepatocellular Carcinoma Cell Proliferation and Tumour ProgressionPLoS One. 8: e82918. 5. Margraf, S. et al. (2015) A polymorphism in a phosphotyrosine signalling motif of CD229 (Ly9, SLAMF3) alters SH2 domain binding and T-cell activation.Immunology. 146 (3): 392-400.
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