|Calculated MW||128955 Da|
|Purification||Purified IgM from tissue culture supernatantPurified IgM from tissue culture supernatant|
|Immunogen||RIL-2 propagated NK1.1+ cells from C57BL/6 mice.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, GPIa, VLA-2 subunit alpha, CD49b, Itga2|
|Target/Specificity||Rat anti-Mouse CD49b monoclonal antibody, clone DX5 recognizes mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells (Pellicciet al.2005).Rat anti-mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Areseet al.2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoliet al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD49b Antibody, clone DX5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Arase, H. et al. (2001) Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).J Immunol. 167 (3): 1141-4. 2. Leong, C.C. et al. (1998) Modulation of natural killer cell cytotoxicity in human cytomegalovirus infection: the role of endogenous class I major histocompatibility complex and a viral class I homolog.J Exp Med. 187 (10): 1681-7. 3. Yin, Y. et al. (2013) Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation.Cancer Immunol Res. 1 (4): 256-68. 4. Lin, C.Y. et al. (2008) Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity.Cancer Lett. 272: 285-95. 5. Thomson, R.C. et al. (2008) Expansion and activation of NK cell populations in a gammaherpesvirus infection.Scand J Immunol. 67: 489-95. 6. Pais, R. et al. (2012) An intracameral injection of antigen induces in situ chemokines and cytokines required for the generation of circulating immunoregulatory monocytes.PLoS One. 7: e43182. 7. Jacobs, N. et al. (2006) Intradermal immune response after infection with Vaccinia virus.J Gen Virol. 87: 1157-61.
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