|Application ||IHC-F, FC, E|
|Calculated MW||71607 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant human MUC18 (D1-D5) Fc protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18|
|Target/Specificity||Mouse anti-Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~:118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmannet al.1989).Mouse anti-Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chenet al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD146 Antibody, clone OJ79c is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. 2. Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.Cell Stem Cell. 3: 301-13. 3. Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.Stem Cells. 26: 2408-2418. 4. Park, T.S. et al. (2010) Placental Perivascular Cells for Human Muscle Regeneration.Stem Cells Dev. 20: 451-63. 5. Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.Immunology. 132: 256-65. 6. James, A.W. et al. (2012) Perivascular stem cells: a prospectively purified mesenchymal stem cell population for bone tissue engineering.Stem Cells Transl Med. 1 (6): 510-9. 7. Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.Cell Microbiol. 13: 198-209. 8. Lee, J.H. et al. (2012) Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells.J Biomed Mater Res A.101: 942-53. 9. Boneberg, E.M. et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process.Microvasc Res. 78: 325-31. 10. Murakami, M. et al. (2012) Identification of novel function of vimentin for quality standard for regenerated pulp tissue.J Endod. 38: 920-6. 11. Nielsen, C.T. et al. (2011) Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus.Arthritis Rheum. 63: 3067-77. 12. Zhang, Y. et al. (2009) The Impact of Proliferative Potential of Umbilical Cord–Derived Endothelial Progenitor Cells and Hypoxia on Vascular Tubule Formation In Vitro.Stem Cells Dev. 18: 359-75. 13. Iversen, L.V. et al. (2013) A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer.J Immunol Methods. 388 (1-2): 49-59. 14. Meireles, A.L. et al. (2011) Increased levels of circulating endothelial progenitor cells in human T-cell lymphotropic virus type I carriers.Arch Med Res. 42: 34-7. 15. Iversen, L.V. et al. (2013) Circulating microparticles and plasma levels of soluble E- and P-selectins in patients with systemic sclerosis.Scand J Rheumatol. 42 (6): 473-82. 16. Murakami, M. et al. (2013) The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.Biomaterials. pii: S0142-9612(13)00942-3. 17. Ruetze, M. et al. (2013) A novel niche for skin derived precursors in non-follicular skin.J Dermatol Sci. 69: 132-9. 18. Dokić, J. et al. (2013) Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.Eur J Immunol. 43: 1862-72. 19. Chen, W.C. et al. (2015) Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity.Stem Cells. 33 (2): 557-73. 20. Iversen, L.V. et al. (2015) Cross-sectional study of soluble selectins, fractions of circulating microparticles and their relationship to lung and skin involvement in systemic sclerosis.BMC Musculoskelet Disord. 16: 191.1. Kuzu, I. et al. (1993) Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.Lab Invest. 69 (3): 322-8.2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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