|Application ||IF, FC, IP|
|Calculated MW||25637 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||HPB-ALL cell line.|
|Shelf Life||18 months from date of despatch.|
|Other Names||CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30|
|Target/Specificity||Mouse anti-Human CD63 antibody, clone MEM-259 recognizes the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60 kDa.CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies (Vischeret al.1993) and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation (Hamamotoet al.1994). CD63 expression is also associated with melanoma development (Radfordet al.1997) and expression is rapidly down-regulated by progesterone in endometrial tissues (Okadaet al.1999).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD63 Antibody, clone MEM-259 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.Mol Cell Proteomics. 6 (5): 767-80. 2. Durand-Panteix, S. et al. (2012) B7-H1, which represses EBV-immortalized B cell killing by autologous T and NK cells, is oppositely regulated by c-Myc and EBV latency III program at both mRNA and secretory lysosome levels.J Immunol. 189 (1): 181-90. 3. Abdel-Latif, D. et al. (2004) Rac2 is critical for neutrophil primary granule exocytosis.Blood. 104: 832-9. 4. Norling, L.V. et al. (2012) Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.Arterioscler Thromb Vasc Biol. 32 (8): 1970-8. 5. McKechnie, N.M. et al. (2006) Fas-ligand is stored in secretory lysosomes of ocular barrier epithelia and released with microvesicles.Exp Eye Res. 83: 304-14. 6. Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.PLoS One. 8(5):e62654. 7. Pliyev, B.K. (2008) Activated human neutrophils rapidly release the chemotactically active D2D3 form of the urokinase-type plasminogen activator receptor (uPAR/CD87).Mol Cell Biochem. 321: 111-22. 8. Suraeva, N.M. et al. (2015) Changes in the Morphological and Immunological Characteristics of Mel Ibr Melanoma Cells in Response to Chicken Embryo Extract.Bull Exp Biol Med. 159 (4): 520-3.
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