|Application ||WB, IHC-F, FC, Func|
|Calculated MW||38154 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from ascites|
|Immunogen||Human leucocytes from individuals with CML.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Carcinoembryonic antigen-related cell adhesion molecule 8, CD67 antigen, Carcinoembryonic antigen CGM6, Non-specific cross-reacting antigen NCA-95, CD66b, CEACAM8, CGM6|
|Target/Specificity||Mouse anti-Human CD66b antibody, clone 80H3 reacts with the CGM6 gene product of the carcinoembryonic gene family. This is a 95-100kDa GPI anchored molecule. CD66b is expressed strongly by mature granulocytes and metamyelocytes, and weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD66b Antibody, clone 80H3 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Mannoni, P. et al. (1982) Monoclonal antibodies against human granulocytes and myeloid differentiation. Hum. Immunol. 5: 309-323. 2. Ionita, M.G. et al. (2010) High neutrophil numbers in human carotid atherosclerotic plaques are associated with characteristics of rupture-prone lesions.Arterioscler Thromb Vasc Biol. 30 (9): 1842-8. 3. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.J. Leukoc. Biol. 87: 905-14. 4. Feuk-Lagerstedt, E. et al. (1999) Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.J Immunol. 163: 5592-8. 5. Jankowski, A. et al. (2002) Determinants of the phagosomal pH in neutrophils.J Biol Chem. 277: 6059-66. 6. Jinnouchi, A. et al. (2005) Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms.J Leukoc Biol. 78: 1356-65. 7. Thickett, D.R. et al. (2002) A role for vascular endothelial growth factor in acute and resolving lung injury.Am J Respir Crit Care Med. 166: 1332-7. 8. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides.J Immunol Methods. 313: 96-109. 9. Ottonello, L. et al. (1999) Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins.Blood. 93: 3505-11. 10. Ottonello, L. et al. (2000) Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis.J Leukoc Biol. 68: 662-8. 11. Moraes, T.J. et al. (2006) Abnormalities in the pulmonary innate immune system in cystic fibrosis.Am J Respir Cell Mol Biol. 34: 364-74. 12. Nijhuis, J. et al. (2009) Neutrophil Activation in Morbid Obesity, Chronic Activation of Acute InflammationObesity (Silver Spring). 17: 2014-8. 13. Pliyev, B.K. and Menshikov, M.Y. (2010) Release of the soluble urokinase-type plasminogen activator receptor (suPAR) by activated neutrophils in rheumatoid arthritis.Inflammation. 33: 1-9. 14. Binet, F. and Girard, D. (2008) Novel human neutrophil agonistic properties of arsenic trioxide: involvement of p38 mitogen-activated protein kinase and/or c-jun NH2-terminal MAPK but not extracellular signal-regulated kinases-1/2.J Leukoc Biol. 84: 1613-22. 15. Rosenkranz, A.R. et al. (1999) Novel C5-dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes.J Am Soc Nephrol. 10: 128-35. 16. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.J Leukoc Biol. 87: 905-14. 17. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slidesJ Immunol Methods. 313: 96-109. 18. Orozco-Uribe, M. et al. (2015) Early Exposure of Human Neutrophils to Mycobacteria Triggers Cell Damage and Pro-Inhibitory Molecules, but not ActivationTuberculosis - Expanding Knowledge. Book edited by Wellman Ribon ISBN 978-953-51-2139-8
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