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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD66b Antibody, clone 80H3 (FITC)   

Anti-Human CD66b Antibody, clone 80H3

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, FC, Func
Primary Accession P31997
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 80H3
Calculated MW 38154 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from ascites
Immunogen Human leucocytes from individuals with CML.
Shelf Life 18 months from date of despatch.
Gene ID 1088
Other Names Carcinoembryonic antigen-related cell adhesion molecule 8, CD67 antigen, Carcinoembryonic antigen CGM6, Non-specific cross-reacting antigen NCA-95, CD66b, CEACAM8, CGM6
Target/Specificity Mouse anti-Human CD66b antibody, clone 80H3 reacts with the CGM6 gene product of the carcinoembryonic gene family. This is a 95-100kDa GPI anchored molecule. CD66b is expressed strongly by mature granulocytes and metamyelocytes, and weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD66b Antibody, clone 80H3 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Mannoni, P. et al. (1982) Monoclonal antibodies against human granulocytes and myeloid differentiation.
Hum. Immunol. 5: 309-323. 2. Ionita, M.G. et al. (2010) High neutrophil numbers in human carotid atherosclerotic plaques are associated with characteristics of rupture-prone lesions.
Arterioscler Thromb Vasc Biol. 30 (9): 1842-8. 3. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
J. Leukoc. Biol. 87: 905-14. 4. Feuk-Lagerstedt, E. et al. (1999) Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.
J Immunol. 163: 5592-8. 5. Jankowski, A. et al. (2002) Determinants of the phagosomal pH in neutrophils.
J Biol Chem. 277: 6059-66. 6. Jinnouchi, A. et al. (2005) Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms.
J Leukoc Biol. 78: 1356-65. 7. Thickett, D.R. et al. (2002) A role for vascular endothelial growth factor in acute and resolving lung injury.
Am J Respir Crit Care Med. 166: 1332-7. 8. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides.
J Immunol Methods. 313: 96-109. 9. Ottonello, L. et al. (1999) Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins.
Blood. 93: 3505-11. 10. Ottonello, L. et al. (2000) Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis.
J Leukoc Biol. 68: 662-8. 11. Moraes, T.J. et al. (2006) Abnormalities in the pulmonary innate immune system in cystic fibrosis.
Am J Respir Cell Mol Biol. 34: 364-74. 12. Nijhuis, J. et al. (2009) Neutrophil Activation in Morbid Obesity, Chronic Activation of Acute Inflammation
Obesity (Silver Spring). 17: 2014-8. 13. Pliyev, B.K. and Menshikov, M.Y. (2010) Release of the soluble urokinase-type plasminogen activator receptor (suPAR) by activated neutrophils in rheumatoid arthritis.
Inflammation. 33: 1-9. 14. Binet, F. and Girard, D. (2008) Novel human neutrophil agonistic properties of arsenic trioxide: involvement of p38 mitogen-activated protein kinase and/or c-jun NH2-terminal MAPK but not extracellular signal-regulated kinases-1/2.
J Leukoc Biol. 84: 1613-22. 15. Rosenkranz, A.R. et al. (1999) Novel C5-dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes.
J Am Soc Nephrol. 10: 128-35. 16. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
J Leukoc Biol. 87: 905-14. 17. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides
J Immunol Methods. 313: 96-109. 18. Orozco-Uribe, M. et al. (2015) Early Exposure of Human Neutrophils to Mycobacteria Triggers Cell Damage and Pro-Inhibitory Molecules, but not Activation
Tuberculosis - Expanding Knowledge. Book edited by Wellman Ribon ISBN 978-953-51-2139-8

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Cat# ABD12175
(40 western blots)
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