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>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine Interleukin-12/23 Antibody, clone CC326    

Anti-Bovine Interleukin-12/23 Antibody, clone CC326 (Biotin)

Mouse Anti-Bovine Monoclonal Antibody

     
  • E - Anti-Bovine Interleukin-12/23 Antibody, clone CC326 (Biotin) ABD12179
    Published customer image:Main cell population producing IL-12 in response to R-848.Neonatal MLN cells were cultured in vitro with or without 0.5 µg/ml R-848 and, at various time points, supernatants were harvested and ELISA was carried out for IL-12. Mean ± SEM IL-12 secretion is shown for each time point (A). Neonatal MLN cells were cultured in vitro with (black bars) or without (hatched bars) 0.5 µg/ml R-848 for 8 h, with brefeldin A added for the last 5 h. Cells were harvested for IL-12 intracellular staining combined with labelling of CD11b, CD14, MHC class II and CD205. Three independent experiments were carried out and the data shown are the mean proportions of IL-12+ cells after gating on CD11b+, CD14+, MHC class II+ or CD205+ MLN cells (B).From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5: e13705.
  • E - Anti-Bovine Interleukin-12/23 Antibody, clone CC326 (Biotin) ABD12179
    Published customer image:MLN and spleen cell IL-12 responses to R-848 stimulation as a function of age. MLN and spleen cells from 6- to 14-day-old neonates (closed circles), 20-day-old lambs (closed diamonds) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 µg/ml R-848. At the end of the culture period, supernatants were harvested and ELISA carried out to assess IL-12 secretion by MLN cells (A) or spleen cells (B). Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates, 20-day-old lambs and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5: e13705.
  • E - Anti-Bovine Interleukin-12/23 Antibody, clone CC326 (Biotin) ABD12179
    Published customer image:Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 µg/ml polyI:C, 0.5 µg/ml R-848 or 5 µg/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5: e13705.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession P54349
Other Accession P46282
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names CC326
Calculated MW 24944 Da
Additional Information
Other Species Sh,H
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Recombinant bovine IL-12.
Shelf Life 18 months from date of despatch.
Gene ID 281856
Other Names Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, IL12A
Target/Specificity Mouse anti-Bovine Interleukin-12/23 antibody, clone CC326 recognizes the p40 subunit of bovine interleukin-12. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. Mouse anti-Bovine Interleukin-12/23 antibody, clone CC326 has been shown to block the biological activity of bovine IL-12.
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine Interleukin-12/23 Antibody, clone CC326 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12.
J. Immunol. Methods. 266: 117 - 126. 2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. 3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. colimastitis.
Funct Integr Genomics. 10: 21-38. 4. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. 5. Contreras, V. et al. (2010) Existence of CD8a-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. 6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. 7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. 8. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
J Virol. 82: 1777-86. 9. Stephens, S.A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
Immunology. 110: 48-57. 10. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res.10: 293. 11. Beechler, B.R. et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).
Proc Biol Sci. 282 (1805): .

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