Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   Anti-Human CD195 Antibody, clone HEK/1/85a (RPE)   

Anti-Human CD195 Antibody, clone HEK/1/85a

Rat Anti-Human Monoclonal Antibody

     
  • WB - Anti-Human CD195 Antibody, clone HEK/1/85a  ABD12180
    Published customer image:Rat anti Human CD195 antibody, clone HEK/1/85a used for the immunoprecipitation of CD195 from transfected CHO lysates.Image caption:Ligand-elicited recruitment of arrestin2 to CCR5. CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with the indicated chemokines (100 nM, 50 min), washed extensively, crosslinked, washed again and lysed prior to immunoprecipitation with an anti-CCR5 antibody. Immunoprecipitates were subjected to Western blot using antibodies against GFP, arrestin2 and CCR5. CP; total cell pellet control, IP; antibody specificity used for immunoprecipitation, IB; antibody specificity used for immunoblot.From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5. PLoS ONE 10: e0125396.
  • FC - Anti-Human CD195 Antibody, clone HEK/1/85a  ABD12180
    Staining of human peripheral blood lymphocytes with Rat anti Human CD195:RPE
  • FC - Anti-Human CD195 Antibody, clone HEK/1/85a  ABD12180
    Staining of human peripheral blood lymphocytes with Rat anti Human CD195: Azide Free
  • FC - Anti-Human CD195 Antibody, clone HEK/1/85a  ABD12180
    Staining of human peripheral blood lymphocytes with Rat anti Human CD195:FITC
  • IF - Anti-Human CD195 Antibody, clone HEK/1/85a  ABD12180
    Published customer image:Rat anti Human CD195 antibody, clone HEK/1/85a used for the detection of CD195 expression by transfected CHO cells by immunofluorescence. Image caption:Spatial and temporal resolution of arrestin2-CCR5 association. A CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with chemokine analogs (100 nM) as indicated and the redistribution of arrestin2-GFP was followed by live fluorescence microscopy. Images captured prior to (0 min) and after ligand treatment (6 min) are shown. B CHO-CCR5 cells stably transfected with arrestin2-GFP , preincubated with rhodamine-labeled anti-CCR5 antibody , were washed and then incubated 90 min at 37°C with chemokine analogs (100 nM) prior to image capture. Maximal intensity projections are shown. Scale bar = 20 µm.From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5. PLoS ONE 10(4): e0125396.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P51681
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names HEK/1/85a
Calculated MW 40524 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen CHO cells transfected with human CCR5.
Shelf Life 18 months from date of despatch.
Gene ID 1234
Other Names C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195, CCR5, CMKBR5
Target/Specificity Rat anti-Human CD195 antibody, clone HEK/1/85a recognizes the human CD195 cell surface antigen, a 45 kDa glycoprotein also known as CCR5.CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and also serves as a co-receptor for the entry of HIV into cells.CD195 is expressed by a subset of T lymphocytes and by monocytes.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD195 Antibody, clone HEK/1/85a is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD12180
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
30% off on 400+ Stem Cells Antibodies. PromoCode: <span class=text-red>FLASH19</span>
Terms & Conditions