|Other Species||Ga,Sh,Red deer,Mule deer|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessenset al.1990). CD21 may be expressed on B cells as either a long or a short form (Pringleet al.2012)Mouse anti-bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with PrPc on B cells of scrapie infected sheep (Hallidayet al.2005).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Bovine CD21 Antibody, clone CC21 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-7. 2. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.Immunology 69: 525-30. 3. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8, and WC1 gamma-delta TCR+ cells from cattle lymph nodes and blood. Vet. Immunol. Immunopathol. 81: 85-96. 4. Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.Cell Tissue Res. 338: 343-54. 5. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.Acta Histochem. 113: 416-22. 6. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.J. Gen. Virol. 86: 1571-9. 7. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.J Virol. 79: 7738-44. 8. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.Comp Immunol Microbiol Infect Dis. 34: 217-25. 9. Richt, J.A. et al. (2007) Production of cattle lacking prion protein.Nat Biotechnol. 25: 132-8. 10. Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.J Infect Dev Ctries. 4: 576-9. 11. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.Vet Res Commun. 34: 197-203. 12. Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow.Vet Rec. 170: 312. 13. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.Vet Immunol Immunopathol. 137: 284-90. 14. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.Vet Pathol. 43: 127-35. 15. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.Vet Immunol Immunopathol. 146: 1-7. 16. Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.J Gen Virol. 83: 2617-28. 17. Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.J Comp Pathol. 146: 365-71. 18. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.Virology. 405: 110-9. 19. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.Theriogenology. 82: 403-10. 20. Meganck, V. et al. (2014) Development of a method for isolating bovine colostrum mononuclear leukocytes for phenotyping and functional studies.Vet J. 200: 294-8.
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