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>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine CD21 Antibody, clone CC21 (RPE)   

Anti-Bovine CD21 Antibody, clone CC21 (FITC)

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti-Bovine CD21 Antibody, clone CC21 (FITC) ABD12184
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC21
Additional Information
Other Species Ga,Sh,Red deer,Mule deer
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessenset al.1990). CD21 may be expressed on B cells as either a long or a short form (Pringleet al.2012)Mouse anti-bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with PrPc on B cells of scrapie infected sheep (Hallidayet al.2005).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Bovine CD21 Antibody, clone CC21 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
Vet. Immunol. Immunopathol. 27: 21-7. 2. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.
Immunology 69: 525-30. 3. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8, and WC1 gamma-delta TCR+ cells from cattle lymph nodes and blood.
Vet. Immunol. Immunopathol. 81: 85-96. 4. Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.
Cell Tissue Res. 338: 343-54. 5. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22. 6. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J. Gen. Virol. 86: 1571-9. 7. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. 8. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
Comp Immunol Microbiol Infect Dis. 34: 217-25. 9. Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
Nat Biotechnol. 25: 132-8. 10. Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.
J Infect Dev Ctries. 4: 576-9. 11. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
Vet Res Commun. 34: 197-203. 12. Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow.
Vet Rec. 170: 312. 13. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
Vet Immunol Immunopathol. 137: 284-90. 14. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35. 15. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. 16. Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.
J Gen Virol. 83: 2617-28. 17. Dagleish, al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.
J Comp Pathol. 146: 365-71. 18. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405: 110-9. 19. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82: 403-10. 20. Meganck, V. et al. (2014) Development of a method for isolating bovine colostrum mononuclear leukocytes for phenotyping and functional studies.
Vet J. 200: 294-8.

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Cat# ABD12184
(40 western blots)
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