|Application ||WB, IHC-F, FC, IP|
|Calculated MW||24551 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified splenic NK cells from the LEW rat strain.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Killer cell lectin-like receptor subfamily B member 1A, NKR-P1A, Antigen 3.2.3, CD161 antigen-like family member A, Natural killer cell surface protein P1-3.2.3, NKR-P1 3.2.3, CD161a, Klrb1a, Nkrp1a|
|Target/Specificity||Mouse anti-Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a single C-type lectin domain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, clone 10/78 recognizes both forms of CD161 (Liet al.2003). Clone 10/78 competes with another anti-CD161 clone, 3.2.3 for binding to antigen.Mouse anti-Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrannet al.2010).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat CD161 Antibody, clone 10/78 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Li, J. et al. (2003) Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B.Int Immunol. 15: 411-6. 2. Sedgwick, J.D. et al. (1998) Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts.J Immunol. 160 (11): 5320-30. 3. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.Mol Vis. 16: 1928-35. 4. Lyons, A. et al. (2011) Atorvastatin prevents age-related and amyloid-beta-induced microglial activation by blocking interferon-gamma release from natural killer cells in the brain.J Neuroinflammation. 8: 27. 5. Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.Cancer Res. 65: 7194-204. 6. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.Br J Ophthalmol. 87: 1515-22. 7. Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.Immunology. 121: 555-64. 8. Tliba, O. et al. (2002) Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats.Vet Res. 33 (3): 327-32. 9. Blöcher, S. et al. (2007) Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.Clin Immunol. 124 (1): 98-108. 10. Koch, M. et al. (2015) Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.Stem Cells International. 2015: 1-7. 11. Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.Cell Mol Immunol. 9 (2): 163-74. 12. Wrann, C.D. et al. (2010) Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.Exp Toxicol Pathol. 62 (1): 1-8. 13. Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.Kidney Int. 58 (1): 100-14. 14. Obara, H. et al. (2005) IFN-gamma, produced by NK cells that infiltrate liver allografts early after transplantation, links the innate and adaptive immune responses.Am J Transplant. 5 (9): 2094-103. 15. Beutel, G. et al. (2013) Effect of chronic elevated asymmetric dimethylarginine (ADMA) levels on granulopoiesis.Ann Hematol. 92 (4): 505-8. 16. Lee, J.S. et al. (2011) Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents.Food Chem. 126 (4): 1521-6. 17. Williamson, L.L. et al. (2015) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection.Brain Behav Immun. pii: S0889-1591(15)00240-8. 18. Arndt, T. et al. (2014) Variable immune cell frequencies in peripheral blood of LEW.1AR1-iddm rats over time compared to other congenic LEW strains.Clin Exp Immunol. 177 (1): 168-78. 19. Kuper, C.F. et al. (2011) Oxazolone (OXA) is a respiratory allergen in Brown Norway rats.Toxicology. 290 (1): 59-68. 20. Arsenović-Ranin, N. et al. (2013) Ovarian hormone withdrawal in prepubertal developmental stage does not prevent thymic involution in rats.Exp Biol Med (Maywood). 238 (6): 641-57. 21. Djikić J et al. (2014) Age-associated changes in rat immune system: lessons learned from experimental autoimmune encephalomyelitis.Exp Gerontol. 58: 179-97. 22. Lemke Anja et al. (2015) Rat renal transplant model for mixed acute humoral and cellular rejection: Weak correlation of serum cytokines/chemokines with intragraft changesTransplant Immunology. pii: S0966-3274(15)30014-9.
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