|Application ||WB, IHC-F, FC, E|
|Calculated MW||54405 Da|
|Purification||Purified IgG prepared by ion exchange chromatography|
|Immunogen||Fusion protein of the extracellular part of the human interferon-gamma receptor (AA: Ala17-Gly245) conjugated to 11 AA of the c-myc proto-oncogene (for affinity purification).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, CD119, IFNGR1|
|Target/Specificity||Mouse anti-Human CD119 antibody, clone BB1E2 recognizes the human gamma-interferon receptor, also known as CD119. CD119 is expressed by monocytes, and very weakly by resting peripheral blood lymphocytes.Mouse anti-Human CD119 antibody, clone BB1E2 recognizes a single protein band of ~43kDa in western blotting under non-reducing conditions against protein comprising the glycosylated extracellular domain of the human interferon-gamma receptor fused to c-myc, and a MW of 27kDa against a recombinant protein corresponding to the non-glycosylated domain fused to c-myc.Mouse anti-Human CD119 antibody, clone BB1E2 does not neutralize biological activity of interferon gamma.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD119 Antibody, clone BB1E2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Entrican, G. et al. (2002) Gamma interferon fails to induce expression of indoleamine 2,3-dioxygenase and does not control the growth of Chlamydophila abortus in BeWo trophoblast cells.Infect. Immun. 70 (5): 2690 - 2693. 2. Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.Vet Immunol Immunopathol. 119: 106-14. 3. López-Herrera, G. et al. (2004) Severe combined immunodeficiency syndrome associated with colonic stenosis.Arch Med Res. 35: 348-58. 4. Garibay-Escobar, A. et al. (2003) Integrated measurements by flow cytometry of the cytokines IL-2, IFN-gamma, IL-12, TNF-alpha and functional evaluation of their receptors in human blood.J Immunol Methods. 280: 73-88. 5. Jankovicova, K. et al. (2012) Interferon gamma receptor expression on granulocytes of cardiac surgical patients is modulated differently by the type of cardiopulmonary bypass used.Perfusion. 27: 49-55. 6. Diaz-Romero, J. et al. (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture.J Cell Physiol. 202: 731-42. 7. Garibay-Escobar, A. et al. (2003) Integrated measurements by flow cytometry of the cytokines IL-2, IFN-gamma, IL-12, TNF-alpha and functional evaluation of their receptors in human blood.J Immunol Methods. 280: 73-88. 8. Ghaemmaghami, A.M. et al. (2002) The proteolytic activity of the major dust mite allergen Der p 1 conditions dendritic cells to produce less interleukin-12: allergen-induced Th2 bias determined at the dendritic cell level.Clin Exp Allergy. 32: 1468-75. 9. Yim, J.H. et al. (2003) The role of interferon regulatory factor-1 and interferon regulatory factor-2 in IFN-gamma growth inhibition of human breast carcinoma cell lines.J Interferon Cytokine Res. 23: 501-11.1. Michiels, L. et al. (1998) The soluble extracellular portion of the human interferon-gamma receptor is a valid substitute for evaluating binding characteristics and for neutralizing the biological activity of this cytokine.Int J Biochem Cell Biol. 30: 505-16.
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