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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD119 Antibody, clone BB1E2 (FITC)   

Anti-Human CD119 Antibody, clone BB1E2

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD119 Antibody, clone BB1E2  ABD12189
    Staining of human peripheral blood monocytes with Mouse anti Human CD119:FITC
  • FC - Anti-Human CD119 Antibody, clone BB1E2  ABD12189
    Staining of human peripheral blood monocytes with Mouse anti Human CD119:Alexa Fluor® 488
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, FC, E
Primary Accession P15260
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names BB1E2
Calculated MW 54405 Da
Additional Information
Other Species B
Purification Purified IgG prepared by ion exchange chromatography
Immunogen Fusion protein of the extracellular part of the human interferon-gamma receptor (AA: Ala17-Gly245) conjugated to 11 AA of the c-myc proto-oncogene (for affinity purification).
Shelf Life 18 months from date of despatch.
Gene ID 3459
Other Names Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, CD119, IFNGR1
Target/Specificity Mouse anti-Human CD119 antibody, clone BB1E2 recognizes the human gamma-interferon receptor, also known as CD119. CD119 is expressed by monocytes, and very weakly by resting peripheral blood lymphocytes.Mouse anti-Human CD119 antibody, clone BB1E2 recognizes a single protein band of ~43kDa in western blotting under non-reducing conditions against protein comprising the glycosylated extracellular domain of the human interferon-gamma receptor fused to c-myc, and a MW of 27kDa against a recombinant protein corresponding to the non-glycosylated domain fused to c-myc.Mouse anti-Human CD119 antibody, clone BB1E2 does not neutralize biological activity of interferon gamma.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD119 Antibody, clone BB1E2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Entrican, G. et al. (2002) Gamma interferon fails to induce expression of indoleamine 2,3-dioxygenase and does not control the growth of Chlamydophila abortus in BeWo trophoblast cells.
Infect. Immun. 70 (5): 2690 - 2693. 2. Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
Vet Immunol Immunopathol. 119: 106-14. 3. López-Herrera, G. et al. (2004) Severe combined immunodeficiency syndrome associated with colonic stenosis.
Arch Med Res. 35: 348-58. 4. Garibay-Escobar, A. et al. (2003) Integrated measurements by flow cytometry of the cytokines IL-2, IFN-gamma, IL-12, TNF-alpha and functional evaluation of their receptors in human blood.
J Immunol Methods. 280: 73-88. 5. Jankovicova, K. et al. (2012) Interferon gamma receptor expression on granulocytes of cardiac surgical patients is modulated differently by the type of cardiopulmonary bypass used.
Perfusion. 27: 49-55. 6. Diaz-Romero, J. et al. (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture.
J Cell Physiol. 202: 731-42. 7. Garibay-Escobar, A. et al. (2003) Integrated measurements by flow cytometry of the cytokines IL-2, IFN-gamma, IL-12, TNF-alpha and functional evaluation of their receptors in human blood.
J Immunol Methods. 280: 73-88. 8. Ghaemmaghami, A.M. et al. (2002) The proteolytic activity of the major dust mite allergen Der p 1 conditions dendritic cells to produce less interleukin-12: allergen-induced Th2 bias determined at the dendritic cell level.
Clin Exp Allergy. 32: 1468-75. 9. Yim, J.H. et al. (2003) The role of interferon regulatory factor-1 and interferon regulatory factor-2 in IFN-gamma growth inhibition of human breast carcinoma cell lines.
J Interferon Cytokine Res. 23: 501-11.1. Michiels, L. et al. (1998) The soluble extracellular portion of the human interferon-gamma receptor is a valid substitute for evaluating binding characteristics and for neutralizing the biological activity of this cytokine.
Int J Biochem Cell Biol. 30: 505-16.

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