|Application ||WB, IHC-P, IHC-F, IF, FC|
|Calculated MW||23147 Da|
|Other Species||B,Dog,Hs,Rh,Pig,C,Raccoon,Great horned owl (Bubo virginianus),Bullfrog,X,Amphibia,Spotted Hyena,Sea Lion,Cat,Amazon Parrot,Mammalian,Birds,M,Duck,Koala,Harbour Porpoise,Alpaca,Cynomolgus Pr|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E|
|Target/Specificity||Rat anti-Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008;Guy and Vignali 2009;Smith-Garvinet al.2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti-Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Joneset al.1993;Kothlowet al.2005)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD3 Antibody, clone CD3-12 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.J Immunol. 150 (12): 5429-35. 2. Shulga-Morskaya, S. et al. (2004) B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation.J Immunol. 173 (4): 2331-41. 3. Kapturczak, M.H. et al. (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse.Am J Pathol. 165 (3): 1045-53. 4. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.Dev Comp Immunol. 29 (8): 733-48. 5. Patole, P.S. et al. (2006) Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice.Nephrol Dial Transplant. 21 (11): 3062-73. 6. Foryst-Ludwig, A. et al. (2010) PPARgamma activation attenuates T-lymphocyte-dependent inflammation of adipose tissue and development of insulin resistance in obese mice.Cardiovasc Diabetol. 9: 64. 7. Osorio, Y. et al. (2011) Identification of small molecule lead compounds for visceral leishmaniasis using a novel ex vivo splenic explant model system.PLoS Negl Trop Dis. 5:e962. 8. Flatz, L. et al (2011) T cell-dependence of Lassa fever pathogenesis.PLoS Pathog. 6: e1000836. 9. Gendronneau, G. et al. (2010) Influence of Hoxa5 on p53 tumorigenic outcome in mice.Am J Pathol. 176: 995-1005. 10. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.Infect Immun. 74: 4841-8. 11. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.Antimicrob Agents Chemother. 56: 687-96. 12. Roy, M. et al. (2012) CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.J Neuroinflammation. 9: 18. 13. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation.J Clin Pathol. 65 (12): 1077-83. 14. Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.Vet Immunol Immunopathol. 147: 35-43. 15. Beineke, A. et al. (2007) Phenotypical characterization of changes in thymus and spleen associated with lymphoid depletion in free-ranging harbor porpoises (Phocoena phocoena).Vet Immunol Immunopathol. 117: 254-65. 16. Pusterla, N. et al. (2006) Multicentric T-cell lymphosarcoma in an alpaca.Vet J. 171: 181-5. 17. Fuller, C.L. et al. (2003) In situ study of abundant expression of proinflammatory chemokines and cytokines in pulmonary granulomas that develop in cynomolgus macaques experimentally infected with Mycobacterium tuberculosis.Infect Immun. 71: 7023-34. 18. Singleton, C.L. et al. (2007) Diagnosis and treatment of chronic T-lymphocytic leukemia in a spotted hyena (Crocuta crocuta).J Zoo Wildl Med. 38: 488-91. 19. Colegrove, K.M. et al. (2010) Polyomavirus infection in a free-ranging California sea lion (Zalophus californianus) with intestinal T-cell lymphoma.J Vet Diagn Invest. 22: 628-32. 20. Steinberg, J.D. and Keating, J.H. (2008) What is your diagnosis? Cervical mass in a cat.Vet Clin Pathol. 37: 323-7. 21. Osofsky, A. et al. (2011) T-cell chronic lymphocytic leukemia in a double yellow-headed Amazon parrot (Amazona ochrocephala oratrix).J Avian Med Surg. 25: 286-94. 22. Giannitti, F. et al. (2014) Temporal and geographic clustering of polyomavirus-associated olfactory tumors in 10 free-ranging raccoons (Procyon lotor).Vet Pathol. 51 (4): 832-45. 23. Malka, S. et al. (2008) Disseminated lymphoma of presumptive T-cell origin in a great horned owl (Bubo virginianus).J Avian Med Surg. 22: 226-33. 24. Bricker, N.K. et al. (2012) Cytochemical and immunocytochemical characterization of blood cells and immunohistochemical analysis of spleen cells from 2 species of frog, Rana (Aquarana) catesbeianaandXenopus laevis.Vet Clin Pathol. 41: 353-61. 25. de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.Histochem Cell Biol. 144 (2): 133-46.1. Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient-derived CD8 T cells.J Virol. 72 (10): 7733-44.2. Burudi, E.M. et al. (2002) Regulation of indoleamine 2,3-dioxygenase expression in simian immunodeficiency virus-infected monkey brains.J Virol. 76 (23): 12233-41.3. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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