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Anti-Human CD3 Antibody, clone CD3-12

Rat Anti-Human Monoclonal Antibody

  • IHC - Anti-Human CD3 Antibody, clone CD3-12  ABD12192
    Published customer image:Rat anti Human CD3 antibody, clone CD3-12 used for the detection of CD3 expressing cells in murine tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Insert image captionFrom: Flatz L, Rieger T, Merkler D, Bergthaler A, Regen T, et al. T Cell-Dependence of Lassa Fever Pathogenesis. PLoS Pathog 6: e1000836.
  • IHC-P - Anti-Human CD3 Antibody, clone CD3-12  ABD12192
    Published customer image:Rat anti Mouse CD3 antibody, clone CD3-12 used dor the detection of CD3 expressing cells in human bone marrow, spleen and appendix by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Localization and expression of CD37 in bone marrow (a–f), spleen (g–l) and appendix (m–r). a, g, m Representative original multispectral image of lymphoid organ stained for CD3 (Warp Red), CD37 (True Blue) and cell nuclei (Nuclear Red). Scale bars = 100 µm. b, h, n Composite RGB image after spectral unmixing of original image (red CD3, blue CD37, green nuclei). c, i, o Image showing scoring of CD3-CD37dim , CD3+CD37dim , CD3+CD37bright or CD3-CD37bright cells. Optical density of CD37 on individual cells in human bone marrow (d), in B cell follicle (blue line) and in T cell zone and in red pulp (red line) in human spleen (j) and in B cell follicle (blue line) and in lamina propria (red line) in human appendix (p). Optical densities were binned per 0.05 and normalized to % of max. Percentage of CD37dim and CD37bright cells in total bone marrow (e) and in the CD3+ cell population in human bone marrow (f). Percentage of total CD37bright cells in B cell follicle and red pulp (k) and in the CD3+ cell population in T cell zone and red pulp (l) in human spleen. Percentage of total CD37bright cells (q) and in the CD3+ cell population (r) in B cell follicle and lamina propria in human appendix. Each dot represents data of one ×20 image from the lymphoid tissue. The red line represents the mean. *P < 0.05, ****P < 0.0001From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015 May 8. [Epub ahead of print]
  • WB - Anti-Human CD3 Antibody, clone CD3-12  ABD12192
    Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with Mouse anti Human CD3 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
  • FC - Anti-Human CD3 Antibody, clone CD3-12  ABD12192
    Staining of human peripheral blood lymphocytes with Rat anti Human CD3 following permeabilisation with Leucoperm
  • E - Anti-Human CD3 Antibody, clone CD3-12  ABD12192
    ELISA analysis of human CD3 expression using Rat anti Human CD3&epsilon;, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . A serum sample is included undiluted
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07766
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names CD3-12
Calculated MW 23147 Da
Additional Information
Other Species B,Dog,Hs,Rh,Pig,C,Raccoon,Great horned owl (Bubo virginianus),Bullfrog,X,Amphibia,Spotted Hyena,Sea Lion,Cat,Amazon Parrot,Mammalian,Birds,M,Duck,Koala,Harbour Porpoise,Alpaca,Cynomolgus Pr
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
Shelf Life 18 months from date of despatch.
Gene ID 916
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Target/Specificity Rat anti-Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008;Guy and Vignali 2009;Smith-Garvinet al.2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti-Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Joneset al.1993;Kothlowet al.2005)
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD3 Antibody, clone CD3-12 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
J Immunol. 150 (12): 5429-35. 2. Shulga-Morskaya, S. et al. (2004) B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation.
J Immunol. 173 (4): 2331-41. 3. Kapturczak, M.H. et al. (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse.
Am J Pathol. 165 (3): 1045-53. 4. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
Dev Comp Immunol. 29 (8): 733-48. 5. Patole, P.S. et al. (2006) Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice.
Nephrol Dial Transplant. 21 (11): 3062-73. 6. Foryst-Ludwig, A. et al. (2010) PPARgamma activation attenuates T-lymphocyte-dependent inflammation of adipose tissue and development of insulin resistance in obese mice.
Cardiovasc Diabetol. 9: 64. 7. Osorio, Y. et al. (2011) Identification of small molecule lead compounds for visceral leishmaniasis using a novel ex vivo splenic explant model system.
PLoS Negl Trop Dis. 5:e962. 8. Flatz, L. et al (2011) T cell-dependence of Lassa fever pathogenesis.
PLoS Pathog. 6: e1000836. 9. Gendronneau, G. et al. (2010) Influence of Hoxa5 on p53 tumorigenic outcome in mice.
Am J Pathol. 176: 995-1005. 10. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
Infect Immun. 74: 4841-8. 11. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
Antimicrob Agents Chemother. 56: 687-96. 12. Roy, M. et al. (2012) CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
J Neuroinflammation. 9: 18. 13. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation.
J Clin Pathol. 65 (12): 1077-83. 14. Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.
Vet Immunol Immunopathol. 147: 35-43. 15. Beineke, A. et al. (2007) Phenotypical characterization of changes in thymus and spleen associated with lymphoid depletion in free-ranging harbor porpoises (Phocoena phocoena).
Vet Immunol Immunopathol. 117: 254-65. 16. Pusterla, N. et al. (2006) Multicentric T-cell lymphosarcoma in an alpaca.
Vet J. 171: 181-5. 17. Fuller, C.L. et al. (2003) In situ study of abundant expression of proinflammatory chemokines and cytokines in pulmonary granulomas that develop in cynomolgus macaques experimentally infected with Mycobacterium tuberculosis.
Infect Immun. 71: 7023-34. 18. Singleton, C.L. et al. (2007) Diagnosis and treatment of chronic T-lymphocytic leukemia in a spotted hyena (Crocuta crocuta).
J Zoo Wildl Med. 38: 488-91. 19. Colegrove, K.M. et al. (2010) Polyomavirus infection in a free-ranging California sea lion (Zalophus californianus) with intestinal T-cell lymphoma.
J Vet Diagn Invest. 22: 628-32. 20. Steinberg, J.D. and Keating, J.H. (2008) What is your diagnosis? Cervical mass in a cat.
Vet Clin Pathol. 37: 323-7. 21. Osofsky, A. et al. (2011) T-cell chronic lymphocytic leukemia in a double yellow-headed Amazon parrot (Amazona ochrocephala oratrix).
J Avian Med Surg. 25: 286-94. 22. Giannitti, F. et al. (2014) Temporal and geographic clustering of polyomavirus-associated olfactory tumors in 10 free-ranging raccoons (Procyon lotor).
Vet Pathol. 51 (4): 832-45. 23. Malka, S. et al. (2008) Disseminated lymphoma of presumptive T-cell origin in a great horned owl (Bubo virginianus).
J Avian Med Surg. 22: 226-33. 24. Bricker, N.K. et al. (2012) Cytochemical and immunocytochemical characterization of blood cells and immunohistochemical analysis of spleen cells from 2 species of frog, Rana (Aquarana) catesbeianaandXenopus laevis.
Vet Clin Pathol. 41: 353-61. 25. de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 144 (2): 133-46.1. Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient-derived CD8 T cells.
J Virol. 72 (10): 7733-44.2. Burudi, E.M. et al. (2002) Regulation of indoleamine 2,3-dioxygenase expression in simian immunodeficiency virus-infected monkey brains.
J Virol. 76 (23): 12233-41.3. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD12192
(40 western blots)
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