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Anti-Human CD3 Antibody, clone MEM-57

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD3 Antibody, clone MEM-57  ABD12193
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:FITC
  • FC - Anti-Human CD3 Antibody, clone MEM-57  ABD12193
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04234
Other Accession P07766, P09693
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names MEM-57
Calculated MW 18930 Da
Additional Information
Other Species Chimpanzee
Purification Purified IgG prepared by affinity chromatography on Protein A.
Shelf Life 18 months from date of despatch.
Gene ID 915
Other Names T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD3d, CD3D, T3D
Target/Specificity Mouse anti-Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either δ-ε or γ-ε subunit complexes (Daveet al.1997). Mouse anti-Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3ε subunit (Transyet al.1989) as it will recognize murine 3D054.8 hybridoma cells tranfected with cDNA to express the human CDε subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells. The TCR complex consists of TCR heterodimers, either α/β or γ/δ expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, γ, δ, ε and ζ responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhnset al.2006Daveet al.1997).Mouse anti-Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batistaet al.2004Brdickováet al.2003). Clone MEM-57 immunoprecipitates proteins of ~22 kDa and more weakly ~28 kDa. from peripheral blood T cell lysates.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD3 Antibody, clone MEM-57 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Folia Biol (Praha). 34 (1): 23-34. 2. Dave, V.P. et al. (1998) Altered functional responsiveness of thymocyte subsets from CD3delta-deficient mice to TCR-CD3 engagement.
Int Immunol. 10: 1481-90. 3. Transy, C. et al. (1989) Most anti-human CD3 monoclonal antibodies are directed to the CD3 epsilon subunit.
Eur J Immunol. 19: 947-50. 4. Amlot, P.L. et al. (1996) Activation antigen expression on human T cells. I. Analysis by two-colour flow cytometry of umbilical cord blood, adult blood and lymphoid tissue.
Clin Exp Immunol. 105: 176-82. 5. Gunnlaugsdottir, B. et al. (2008) Naive human T-cells become non-responsive towards anti-TNFalpha (infliximab) treatment in vitro if co-stimulated through CD28.
Scand J Immunol. 68: 624-34. 6. Morris, R.J. et al. (2005) A high-efficiency system of natural killer cell cloning.
J Immunol Methods. 307: 24-33.

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Cat# ABD12193
(40 western blots)
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