|Application ||FC, IP|
|Other Accession||P07766, P09693|
|Calculated MW||18930 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD3d, CD3D, T3D|
|Target/Specificity||Mouse anti-Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either δ-ε or γ-ε subunit complexes (Daveet al.1997). Mouse anti-Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3ε subunit (Transyet al.1989) as it will recognize murine 3D054.8 hybridoma cells tranfected with cDNA to express the human CDε subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells. The TCR complex consists of TCR heterodimers, either α/β or γ/δ expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, γ, δ, ε and ζ responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhnset al.2006Daveet al.1997).Mouse anti-Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batistaet al.2004Brdickováet al.2003). Clone MEM-57 immunoprecipitates proteins of ~22 kDa and more weakly ~28 kDa. from peripheral blood T cell lysates.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD3 Antibody, clone MEM-57 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).Folia Biol (Praha). 34 (1): 23-34. 2. Dave, V.P. et al. (1998) Altered functional responsiveness of thymocyte subsets from CD3delta-deficient mice to TCR-CD3 engagement.Int Immunol. 10: 1481-90. 3. Transy, C. et al. (1989) Most anti-human CD3 monoclonal antibodies are directed to the CD3 epsilon subunit.Eur J Immunol. 19: 947-50. 4. Amlot, P.L. et al. (1996) Activation antigen expression on human T cells. I. Analysis by two-colour flow cytometry of umbilical cord blood, adult blood and lymphoid tissue.Clin Exp Immunol. 105: 176-82. 5. Gunnlaugsdottir, B. et al. (2008) Naive human T-cells become non-responsive towards anti-TNFalpha (infliximab) treatment in vitro if co-stimulated through CD28.Scand J Immunol. 68: 624-34. 6. Morris, R.J. et al. (2005) A high-efficiency system of natural killer cell cloning.J Immunol Methods. 307: 24-33.
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