|Application ||WB, FC, E, IP|
|Calculated MW||40076 Da|
|Purification||Purified IgG prepared by caprylic acid and ammonium sulfate precipitation.|
|Immunogen||Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14|
|Target/Specificity||Mouse anti-Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti-CD14 antibody, clone UCHM1 as a detection reagentMouse anti-Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prageret al.2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandiet al.2007).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human CD14 Antibody, clone MEM-18 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Plötz, S.G. et al. (2001) The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent.Blood. 97 (1): 235-41. 2. Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.J Immunol. 166 (4): 2364-71. 3. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.Vet Immunol Immunopathol. 80 (1-2): 93-109. 4. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. 5. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.J Immunol. 176: 5730-4. 6. Shao, D.D. et al. (2008) Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation.J Leukoc Biol. 83: 1323-33. 7. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012: 172420. 8. Jafarshad, A. et al. (2007) A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII.J Immunol. 178: 3099-106. 9. Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.Ann Biomed Eng. 41 (10): 2181-92.
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