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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD14 Antibody, clone MEM-18 (RPE)   

Anti-Human CD14 Antibody, clone MEM-18

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD14 Antibody, clone MEM-18  ABD12194
    Published customer image:FITC conjugated Mouse anti Human CD14 antibody, clone MEM-18 used for the detection of monocytes in PBMCs by flow cytometry.Image caption:Accessory Cells Are Activated by iRBCs PBMCs from ten malaria-naïve donors were cultured with live iRBCs or E. coli LPS for 24 h or left unstimulated. Surface expression of HLA-DR, CD40, and CD80 on accessory cells was analysed by flow cytometry. Accessory cells were defined as HLA-DR+ CD11c+ cells (A, left), and CD14+ monocytes and CD14- mDCs were selected for further analysis (A, right). Representative examples of surface expression levels of CD40, HLA-DR, and CD80 on HLA-DR+ CD11c+ CD14+ monocytes (B) and HLA-DR+ CD11c+ CD14- mDCs (C) are shown. Data are based on the collection of 100,000 total events. Results for all ten donors are summarised in (D). *, p < 0.05; **, p < 0.01; ***, p < 0.001; p-values are for paired t tests comparing stimulated and unstimulated cells from the same donor.From: Newman KC, Korbel DS, Hafalla JC, Riley EM Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-? Responses to Malaria. PLoS Pathog 2: e118.
  • E - Anti-Human CD14 Antibody, clone MEM-18  ABD12194
    Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone T&uuml;k4 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively
  • FC - Anti-Human CD14 Antibody, clone MEM-18  ABD12194
    Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC
  • E - Anti-Human CD14 Antibody, clone MEM-18  ABD12194
    Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively
  • FC - Anti-Human CD14 Antibody, clone MEM-18  ABD12194
    Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E, IP
Primary Accession P08571
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MEM-18
Calculated MW 40076 Da
Additional Information
Purification Purified IgG prepared by caprylic acid and ammonium sulfate precipitation.
Immunogen Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.
Shelf Life 12 months from date of reconstitution.
Gene ID 929
Other Names Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Target/Specificity Mouse anti-Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti-CD14 antibody, clone UCHM1 as a detection reagentMouse anti-Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prageret al.2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandiet al.2007).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Storage at 4℃ or at -20℃.
PrecautionsAnti-Human CD14 Antibody, clone MEM-18 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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References

1. Plötz, S.G. et al. (2001) The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent.
Blood. 97 (1): 235-41. 2. Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71. 3. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. 4. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. 5. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
J Immunol. 176: 5730-4. 6. Shao, D.D. et al. (2008) Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation.
J Leukoc Biol. 83: 1323-33. 7. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012: 172420. 8. Jafarshad, A. et al. (2007) A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII.
J Immunol. 178: 3099-106. 9. Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Ann Biomed Eng. 41 (10): 2181-92.

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