|Application ||WB, IHC-P, IHC-F, FC, E|
|Calculated MW||48804 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39|
|Target/Specificity||Mouse anti-c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines (Craiget al.1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbitet al.1999).Mouse anti-c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpertet al.2001).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-c-Myc Antibody, clone 9E10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product.Mol. Cell. Biol. 5: 3610 -3616. 2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 7: 1299 -1304. 3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.Microb. Cell Fact. 9: 74-86. 4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.Biochem J. 404: 23-9. 5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.J Cell Biol. 187: 959-66. 6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.Protein Eng. 14: 803-6. 7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.Proteins. 73: 552-65. 8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.J Immunol. 184: 6359-66. 9. Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.J Biol Chem. 285: 26878-88. 10. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.Blood. 106: 2936-43. 11. Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.Mol Biol Cell. 21: 2996-3006. 12. Rowshanravan, B. et al. (2014) RasGAP mediates neuronal survival in Drosophila through direct regulation of Rab5-dependent endocytosis.J Cell Sci. 127: 2849-61. 13. Taylor K et al. (2015) Nanocell targeting using engineered bispecific antibodies.MAbs. 7 (1): 53-65.1. Nesbit, C. et al. (1999) MYC oncogenes and human neoplastic disease.Oncogene. 18: 3004-16.
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