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>   home   >   Products   >   Primary Antibodies   >   Anti-Sheep CD4 Antibody, clone 44.38 (RPE)   

Anti-Sheep CD4 Antibody, clone 44.38 (FITC)

Mouse Anti-Sheep Monoclonal Antibody

  • FC - Anti-Sheep CD4 Antibody, clone 44.38 (FITC) ABD12199
    Published customer image:Mouse anti Sheep CD4 antibody, clone 44.38 used for the detection of CD4 expressing cells in lymphoid tissues by immumnofluorescence microscopy.Image caption:Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
  • FC - Anti-Sheep CD4 Antibody, clone 44.38 (FITC) ABD12199
    Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD4 Alexa Fluor® 647
  • FC - Anti-Sheep CD4 Antibody, clone 44.38 (FITC) ABD12199
    Published customer image:Mouse anti Sheep CD4 antibody, clone 44.38 used for the evaluation of CD4 expression on ovine peripheral blood mononuclear cells by flow cytometry.Image caption:Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05542
Reactivity Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 44.38
Calculated MW 1928 Da
Additional Information
Other Species Ga
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Fetal thymocytes.
Shelf Life 18 months from date of despatch.
Other Names T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4
Target/Specificity Mouse anti-Sheep CD4 antibody, clone 44.38 recognizes the ovine CD4 cell surface glycoprotein, which is expressed by a subset of mature T lymphocytes. Mouse anti-Sheep CD4 antibody, clone 44.38 immunoprecipitates a protein of ~56 kDa under reducing conditions.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Sheep CD4 Antibody, clone 44.38 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Maddox, J. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Immunology. 55: 739 - 748. 2. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur. J. Immunol. 16: 19 - 25. 3. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset .
Cell. Immunol. 110: 46 - 55. 4. Mackay, C. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J. Immunol. 136 (5): 1592 - 1599. 5. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22. 6. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. 7. Brown, M.N. et al. (2010) Chemoattractant receptors and lymphocyte egress from extralymphoid tissue: changing requirements during the course of inflammation.
J Immunol. 185: 4873-82. 8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72. 9. Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339: 1-10. 10. Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
Nat Immunol. 6: 889-94. 11. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17. 12. Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models.
J Virol. 86: 2056-66. 13. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. 14. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium aviumsubsp.paratuberculosis.
J Comp Pathol. 148: 157-72. 15. Umeshappa, C.S. et al. (2010) Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
Vaccine. 28: 2522-31. 16. Kalyanasundaram, A. et al. (2015) Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.
Exp Parasitol. 154: 98-107.1. Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11.

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Cat# ABD12199
(40 western blots)
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