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>   home   >   Products   >   Primary Antibodies   >   Anti-Sheep CD4 Antibody, clone 44.38 (RPE)   

Anti-Sheep CD4 Antibody, clone 44.38 (FITC)

Mouse Anti-Sheep Monoclonal Antibody

  • FC - Anti-Sheep CD4 Antibody, clone 44.38 (FITC) ABD12199
    Published customer image:Mouse anti Sheep CD4 antibody, clone 44.38 used for the detection of CD4 expressing cells in lymphoid tissues by immumnofluorescence microscopy.Image caption:Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
  • FC - Anti-Sheep CD4 Antibody, clone 44.38 (FITC) ABD12199
    Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD4 Alexa Fluor® 647
  • FC - Anti-Sheep CD4 Antibody, clone 44.38 (FITC) ABD12199
    Published customer image:Mouse anti Sheep CD4 antibody, clone 44.38 used for the evaluation of CD4 expression on ovine peripheral blood mononuclear cells by flow cytometry.Image caption:Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05542
Reactivity Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 44.38
Additional Information
Other Species Ga
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Fetal thymocytes.
Shelf Life 18 months from date of despatch.
Other Names T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4
Target/Specificity Mouse anti-Sheep CD4 antibody, clone 44.38 recognizes the ovine CD4 cell surface glycoprotein, which is expressed by a subset of mature T lymphocytes. Mouse anti-Sheep CD4 antibody, clone 44.38 immunoprecipitates a protein of ~56 kDa under reducing conditions.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Sheep CD4 Antibody, clone 44.38 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Maddox, J. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Immunology. 55: 739 - 748. 2. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur. J. Immunol. 16: 19 - 25. 3. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset .
Cell. Immunol. 110: 46 - 55. 4. Mackay, C. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J. Immunol. 136 (5): 1592 - 1599. 5. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22. 6. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. 7. Brown, M.N. et al. (2010) Chemoattractant receptors and lymphocyte egress from extralymphoid tissue: changing requirements during the course of inflammation.
J Immunol. 185: 4873-82. 8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72. 9. Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339: 1-10. 10. Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
Nat Immunol. 6: 889-94. 11. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17. 12. Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models.
J Virol. 86: 2056-66. 13. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. 14. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium aviumsubsp.paratuberculosis.
J Comp Pathol. 148: 157-72. 15. Umeshappa, C.S. et al. (2010) Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
Vaccine. 28: 2522-31. 16. Kalyanasundaram, A. et al. (2015) Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.
Exp Parasitol. 154: 98-107.1. Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11.

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$ 392.00
Cat# ABD12199
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