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>   home   >   Products   >   Primary Antibodies   >   Anti-Sheep CD8 Antibody, clone 38.65 (RPE)   

Anti-Sheep CD8 Antibody, clone 38.65 (FITC)

Mouse Anti-Sheep Monoclonal Antibody

     
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 38.65
Additional Information
Other Species B,Ga
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Ovine efferent lymphocytes.
Shelf Life 12 months from date of reconstitution.
Target/Specificity Mouse anti-Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.Under reducing conditions, the antigens immunoprecipitated by Mouse anti-Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Sheep CD8 Antibody, clone 38.65 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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References

1. Maddox, J. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Immunology. 55: 739 748. 2. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur. J. Immunol. 16: 19 - 25. 3. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell. Immunol. 110: 46 -55. 4. Mackay, C. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.
Eur J Immunol 19: 1477 - 1483. 5. Mackay, C. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J. Immunol. 136 (5): 1592 - 1599 6. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113(4):416-22 7. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11. 8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72. 9. Davies, M.L. et al. (2004) Architecture of secondary lymphoid tissue in sheep experimentally challenged with scrapie.
Immunology. 111: 230-6. 10. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41: 4. 11. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin Vaccine Immunol. 16: 1003-11. 12. Kallapur, S.G. et al. (2011) Pulmonary and Systemic Inflammatory Responses to Intraamniotic IL-1 alpha in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301(3):L285-95 13. Bruce, C.J. et al. (1999) Depletion of bovine CD8+ T cells with chCC63, a chimaeric mouse-bovine antibody.
Vet Immunol Immunopathol. 71 (3-4): 215-31. 14. Nfon, C.K.et al (2012) Innate Immune Response to Rift Valley Fever Virus in Goats.
PLoS Negl Trop Dis.6 (4): e1623. 15. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
J Comp Pathol. 148: 157-72. 16. Olsen, L. et al. (2015) The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1(+) NK cells and by NCR1(-) CD8(+) cell recruitment.
Vet Res. 46: 28.

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Cat# ABD12200
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