|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Ovine efferent lymphocytes.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.Under reducing conditions, the antigens immunoprecipitated by Mouse anti-Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Sheep CD8 Antibody, clone 38.65 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Maddox, J. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies. Immunology. 55: 739 748. 2. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol. 16: 19 - 25. 3. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55. 4. Mackay, C. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development. Eur J Immunol 19: 1477 - 1483. 5. Mackay, C. et al. (1986) Thymocyte subpopulations during early fetal development in sheep. J. Immunol. 136 (5): 1592 - 1599 6. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.Acta Histochem. 113(4):416-22 7. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.Clin. Vaccine. Immunol. 16: 1003-11. 8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.Immunology. 107: 366-72. 9. Davies, M.L. et al. (2004) Architecture of secondary lymphoid tissue in sheep experimentally challenged with scrapie.Immunology. 111: 230-6. 10. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.Vet Res. 41: 4. 11. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.Clin Vaccine Immunol. 16: 1003-11. 12. Kallapur, S.G. et al. (2011) Pulmonary and Systemic Inflammatory Responses to Intraamniotic IL-1 alpha in fetal sheep.Am J Physiol Lung Cell Mol Physiol. 301(3):L285-95 13. Bruce, C.J. et al. (1999) Depletion of bovine CD8+ T cells with chCC63, a chimaeric mouse-bovine antibody.Vet Immunol Immunopathol. 71 (3-4): 215-31. 14. Nfon, C.K.et al (2012) Innate Immune Response to Rift Valley Fever Virus in Goats.PLoS Negl Trop Dis.6 (4): e1623. 15. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.J Comp Pathol. 148: 157-72. 16. Olsen, L. et al. (2015) The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1(+) NK cells and by NCR1(-) CD8(+) cell recruitment.Vet Res. 46: 28.
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