|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Ovine efferent lymphatic duct lymphocytes.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Sheep CD45 antibody, clone 1.11.32 recognizes the ovine CD45 (Leucocyte common antigen), expressed on all ovine lymphocytes, macrophages and granulocytes. Mouse anti-Sheep CD45 antibody, clone 1.11.32 immunoprecipitates CD45 molecules of 190 kDa, 210 kDa and 225 kDa from lymph node lysates.|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Sheep CD45 Antibody, clone 1.11.32 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.Cell Immunol. 110 (1): 46-55. 2. Zannettino, A.C. et al. (2010) Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.Tissue Eng Part A. 16 (12): 3579-87. 3. Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.Eur J Immunol. 19 (8): 1477-83. 4. Maddox, J.F. et al. (1985) The sheep analogue of leucocyte common antigen (LCA).Immunology. 55 (2): 347-53. 5. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.Acta Histochem. 113 (4): 416-22. 6. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.Comp Immunol Microbiol Infect Dis. 34: 217-25. 7. Herdrich, B.J. et al. (2010) Regenerative healing following foetal myocardial infarction.Eur J Cardiothorac Surg. 38: 691-8. 8. Reichert, J.C. et al. (2010) Ovine bone- and marrow-derived progenitor cells and their potential for scaffold-based bone tissue engineering applications in vitro and in vivo.J Tissue Eng Regen Med. 4: 565-76. 9. Galinsky, R. et al. (2011) Effect of intra-amniotic lipopolysaccharide on nephron number in preterm fetal sheep.Am J Physiol Renal Physiol. 301 (2): F280-5. 10. Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1a in fetal sheep.Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. 11. Stickler, P. et al. (2010) Cyclically stretching developing tissue in vivo enhances mechanical strength and organization of vascular grafts.Acta Biomater. 6 (7): 2448-56. 12. Berardinelli, P. et al. (2013) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation.Clin Oral Investig. 17 (7): 1661-75. 13. Geherin, S.A. et al. (2012) The skin, a novel niche for recirculating B cells.J Immunol. 188: 6027-35. 14. Jahroomishirazi, R. et al. (2014) Isolation and Characterization of CD271+ Stem Cells Derived from Sheep Dermal Skin.Cells Tissues Organs. 200 (2): 141-52. 15. Stephens, E.H. et al. (2015) Extracellular matrix remodeling in wound healing of critical size defects in the mitral valve leaflet.Heart Vessels. Nov 12. [Epub ahead of print]
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