|Application ||IHC-F, FC, IP|
|Calculated MW||112678 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified murine platelets.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41, Integrin alpha-IIb heavy chain, Integrin alpha-IIb light chain, Itga2b|
|Target/Specificity||Rat anti-Mouse CD41 antibody, clone MWReg30 recognizes the mouse integrin alpha IIb subunit CD41. CD41 is a ~125kDa single pass type 1 transmembrane glycoprotein expressed by platelets, megakaryocytes (Zhanget al.2007), mast cells (Berlangaet al.2005)and hematopoietic progenitors (Mitjavila-Garciaet al.2002). CD41 forms a heterodimer with CD61. The CD41/CD61 complex is important for platelet adhesion and aggregation (Patelet al.2003) acting as a receptor for many extracellular matrix proteins including fibronectin, thrombospondin and vitronectin (Weiselet al.1992).Rat anti-mouse CD41, clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo(Nieswandtet al.1999).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD41 Antibody, clone MWReg30 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Nieswandt, B. et al. (1999) Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice.Blood. 94 (2): 684-93. 2. Winter, O. et al. (2010) Megakaryocytes constitute a functional component of a plasma cell niche in the bone marrow.Blood. 116: 1867-75. 3. Tamagawa-Mineoka, R. et al. (2007) The role of platelets in leukocyte recruitment in chronic contact hypersensitivity induced by repeated elicitation.Am J Pathol. 170: 2019-29. 4. Takayama, M. et al. (2010) Genetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis.Mol Cell Biol. 30: 2668-80. 5. Larson, M.K. and Watson, S.P. (2006) Regulation of proplatelet formation and platelet release by integrin alpha IIb beta3.Blood. 108: 1509-14. 6. Zanzinger, K. et al. (2009) Regulation of triggering receptor expressed on myeloid cells 1 expression on mouse inflammatory monocytes.Immunology. 128: 185-95. 7. Lutskiy, M.I. et al. (2007) WASP localizes to the membrane skeleton of platelets.Br J Haematol. 139: 98-105. 8. Sullivan, B.P. et al. (2010) Protective and damaging effects of platelets in acute cholestatic liver injury revealed by depletion and inhibition strategies.Toxicol Sci. 115: 286-94. 9. Fujita, R. et al. (2013) NF-E2 p45 Is Important for Establishing Normal Function of Platelets.Mol Cell Biol. 33: 2659-70. 10. Perez, L.E. et al. (2008) SH2-inositol phosphatase 1 negatively influences early megakaryocyte progenitors.PLoS One. 3: e3565. 11. Teeling,,J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interactionActa Neuropathol. 124: 479-90. 12. Motohashi, H. et al. (2010) NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation.Blood. 115 (3): 677-86. 13. Flierl, U. et al. (2015) Phosphorothioate backbone modifications of nucleotide-based drugs are potent platelet activators.J Exp Med. 212 (2): 129-37. 14. Devanathan, V. et al. (2015) Platelet Gi protein Gαi2 is an essential mediator of thrombo-inflammatory organ damage in mice.Proc Natl Acad Sci U S A. 112 (20): 6491-6. 15. Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.Exp Hematol. 43: 891-900. 16. Goggs, R. et al. (2013) The small GTPase Rif is dispensable for platelet filopodia generation in mice.PLoS One. 8 (1): e54663. 17. Williams CM et al. (2015) Identification of roles for the SNARE-associated protein, SNAP29, in mouse platelets.Platelets. Nov 20. [Epub ahead of print] 18. Cuccurullo, A. et al. (2016) Blockade of Thrombopoietin Reduces Organ Damage in Experimental Endotoxemia and Polymicrobial Sepsis.PLoS One. 11 (3): e0151088. 18. Cuccurullo, A. et al. (2016) Blockade of Thrombopoietin Reduces Organ Damage in Experimental Endotoxemia and Polymicrobial Sepsis.PLoS One. 11 (3): e0151088.
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