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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD226 Antibody, clone DX11    

Anti-Human CD226 Antibody, clone DX11

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Published customer image:Mouse anti Human CD226 antibody, clone DX11 used for blocking DNAM-1 on human NK cells to evaluate the role of DNAM-1 in NK cell mediated NK cytotoxicity. Image caption:NO exposed SKO-007 cells enhances NK cell-mediated cytotoxicity. A) NK cells prepared from PBMCs of healthy donors, were incubated with SKO-007 cells, untreated or treated with DETA-NO for 48 h, and used as target cells in a degranulation assay. The assay was performed at the effector:target (E:T) ratio of 2.5:1. After 2 hours at 37°C, cells were stained with anti-CD56, anti-CD3 and anti-CD107a mAbs. Cell surface expression of CD107a was analyzed on CD56+CD3- cells. In order to evaluate the role of DNAM-1, the assay was performed in parallel treating NK cells with blocking anti-DNAM-1 antibody. Results are representative of one out of three independent experiments. B) The MFI of CD107 were calculated based on at least three independent experiments and evaluated by paired Student t test (*P?
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226 : Low Endotoxin
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:RPE
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:FITC
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:Azide Free
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15762
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DX11
Calculated MW 38614 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Human cytotoxic T lymphocyte clone.
Shelf Life 18 months from date of despatch.
Gene ID 10666
Other Names CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226, DNAM1
Target/Specificity Mouse anti-Human CD226 antibody, clone DX11 recognizes human CD226, a ~65 kDa glycoprotein, also known as DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes.Mouse anti-Human CD226 antibody, clone DX11 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojimaet al.2003).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD226 Antibody, clone DX11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Shibuya, A. et al. (1996) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes.
Immunity. 4 (6): 573-81. 2. Shibuya, A. et al. (1998) Protein kinase C is involved in the regulation of both signaling and adhesion mediated by DNAX accessory molecule-1 receptor.
J Immunol. 161 (4): 1671-6. 3. Kojima, H. et al. (2003) CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells.
J Biol Chem. 278 (38): 36748-53. 4. Manes, T.D. and Pober, J.S. (2011) Identification of Endothelial Cell Junctional Proteins and Lymphocyte Receptors Involved in Transendothelial Migration of Human Effector Memory CD4+ T Cells.
J Immunol. 186: 1763-8. 5. Ardolino, M. et al. (2011) DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
Blood. 117: 4778-86. 6. Soriani, A. et al. (2009) ATM-ATR-dependent up-regulation of DNAM-1 and NKG2D ligands on multiple myeloma cells by therapeutic agents results in enhanced NK-cell susceptibility and is associated with a senescent phenotype.
Blood. 113: 3503-11. 7. Matusali, G. et al. (2012) The Human Immunodeficiency Virus Type 1 Nef and Vpu Proteins Downregulate the Natural Killer Cell-Activating Ligand PVR.
J Virol. 86: 4496-504. 8. Fionda, C. et al. (2015) Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.
BMC Cancer. 15 (1): 17.

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Cat# ABD12214
(40 western blots)
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