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Anti-Rituximab Antibody, clone MB2A4

  • E - Anti-Rituximab Antibody, clone MB2A4  ABD12216
    Detection of Rituximab in direct ELISA. Rituximab was used as the coating reagent followed by Rat anti Rituximab:HRP as the detection antibody
  • E - Anti-Rituximab Antibody, clone MB2A4  ABD12216
    Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti Rituximab was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody ABD12216. Data are shown as the mean of three independent measurements
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names MB2A4
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen F(ab)2 fragment of Rituximab.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti-rituximab antibody, clone MB2A4, specifically recognizes the idiotypic determinants expressed by the rituximab chimeric monoclonal antibody. Rituximab is a therapeutic reagent directed against the human CD20 antigen expressed on the surface of B-cells.Rat anti-rituximab antibody, clone MB2A4, is specific for rituximab and does not recognize other CD20 antibodies. Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy (Cragget al.2004andHampsonet al.2010). Clone MB2A4 has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the anti-rituximab antibody to cell bound rituximab (Beumet al.2004).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rituximab Antibody, clone MB2A4 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Cragg, M. S. et al. (2004) A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells.
Blood. 104:2540-2 2. Cragg, M.S. et al. (2004) Apparent modulation of CD20 by rituximab: an alternative explanation.
Blood. 103 (10): 3989-90; author reply 3990-1. 3. Beum, P.V. et al. (2004) Complement activation and C3b deposition on rituximab-opsonized cells substantially blocks binding of phycoerythrin-labeled anti-mouse IgG probes to rituximab.
J Immunol Methods. 294 (1-2): 37-42. 4. Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects.
J Immunol Methods. 360 (1-2): 30-8. 5. Blasco, H. et al. (2007) Evaluation of a peptide ELISA for the detection of rituximab in serum.
J Immunol Methods.325: 127-39. 6. Daydé, D. et al. (2009) Tumor burden influences exposure and response to rituximab: pharmacokinetic-pharmacodynamic modeling using a syngeneic bioluminescent murine model expressing human CD20.
Blood. 113: 3765-72. 7. Aung, T. et al. (2011) Exosomal evasion of humoral immunotherapy in aggressive B-cell lymphoma modulated by ATP-binding cassette transporter A3.
Proc Natl Acad Sci U S A. 108: 15336-41. 8. Schmidt, E. et al. (2009) Immunogenicity of rituximab in patients with severe pemphigus.
Clin Immunol. 132: 334-41. 9. McDonald, V. et al. (2010) Rituximab pharmacokinetics during the management of acute idiopathic thrombotic thrombocytopenic purpura.
J Thromb Haemost. 8: 1201-8. 10. Kagan, L. et al. (2012) Subcutaneous Absorption of Monoclonal Antibodies: Role of Dose, Site of Injection, and Injection Volume on Rituximab Pharmacokinetics in Rats.
Pharm Res. 29: 490-499 11. Kagan, L. and Mager, D.E. (2013) Mechanisms of subcutaneous absorption of rituximab in rats.
Drug Metab Dispos. 41: 248-55. 12. Liu, X.F. et al. (2012) Validation of a Gyrolab™ assay for quantification of rituximab in human serum.
J Pharmacol Toxicol Methods. 65: 107-14. 13. Kagan, L. et al. (2014) Interspecies pharmacokinetic modeling of subcutaneous absorption of rituximab in mice and rats.
Pharm Res. 31: 3265-73. 14. Blasco, H. et al. (2009) Pharmacokinetics of rituximab associated with CHOP chemotherapy in B-cell non-Hodgkin lymphoma.
Fundam Clin Pharmacol. 23: 601-8. 15. Pers, J.O. et al. (2007) BAFF-modulated repopulation of B lymphocytes in the blood and salivary glands of rituximab-treated patients with Sjögren's syndrome.
Arthritis Rheum. 56: 1464-77. 16. Vacher, P. et al. (2015) Localized Store-Operated Calcium Influx Represses CD95-Dependent Apoptotic Effects of Rituximab in Non-Hodgkin B Lymphomas.
J Immunol. pii: 1402942. 17. Komori, M. et al. (2016) Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis
Annals of Clinical and Translational Neurology. Feb 1. [Epub ahead of print]

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Cat# ABD12216
(40 western blots)
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