|Application ||IHC-F, FC, IP|
|Calculated MW||27420 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1, Clec7a, Bgr, Clecsf12, Dectin1|
|Target/Specificity||Rat anti-Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. Rat anti-Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse Dectin-1 Antibody, clone 2A11 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Brown, G. D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.J. Exp. Med. 196:407-412. 2. Taylor, P. R. et al. (2002) The beta-glucan receptor, Dectin-1 is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.J. Immunol. 169:3876-3882. 3. Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.J. Leuk. Biol. 76:86-94. 4. Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 106:2543-2550. 5. Lefevre,L. et al. (2010) PPARc Ligands Switched High Fat Diet-Induced Macrophage M2b Polarization toward M2a Thereby Improving Intestinal Candida EliminationPLoS One. 5(9):e12828. 6. Fei, M. et al. (2011) TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection.Proc Natl Acad Sci U S A. 108 (13): 5360-5. 7. Gazi, U. et al. (2011) Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement.J Biol Chem. 286: 7822-9. 8. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.FASEB J. 25 (6): 1972-82. 9. Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.PLoS Negl Trop Dis. 4: e689. 10. Galès, A. et al. (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.PLoS Pathog. 6: e1000714. 11. Coates, P.J. et al. (2008) Indirect macrophage responses to ionizing radiation: implications for genotype-dependent bystander signaling.Cancer Res. 68: 450-6. 12. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.J Immunol. 181: 6514-24. 13. Hohl, T.M. (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure.J Infect Dis. 198: 176-85. 14. Palma, A.S. et al. (2006) Ligands for the beta-glucan receptor, Dectin-1, assigned using"designer"microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides.J Biol Chem. 281: 5771-9. 15. Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.J Clin Invest. 121: 985-97. 16. Anandasabapathy, N. et al. (2011) Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain.J Exp Med. 208 (8): 1695-705. 17. Takahara, K. et al. (2012) Efficient capture of Candida albicans and zymosan by SIGNR1 augments TLR2-dependent TNF-a production.Int Immunol. 24 (2): 89-96. 18. Fransen, F. et al. (2015) BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.Immunity. pii: S1074-7613(15)00343-X.
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