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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD107b Antibody, clone M3/84 (RPE)   

Anti-Mouse CD107b Antibody, clone M3/84

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD107b Antibody, clone M3/84  ABD12228
    Staining of mouse peritoneal macrophages with Alexa Fluor® 488 conjugated Rat anti Mouse CD107b . Permeabilised with Leucoperm
  • WB - Anti-Mouse CD107b Antibody, clone M3/84  ABD12228
    Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha is included as a loading control. Detection is with Dylight®800 conjugated Goat anti Rat IgG:
  • FC - Anti-Mouse CD107b Antibody, clone M3/84  ABD12228
    Staining of mouse peritoneal macrophages with FITC conjugated Rat anti Mouse CD107b following permeabilisation with Leucoperm
  • FC - Anti-Mouse CD107b Antibody, clone M3/84  ABD12228
    Staining of mouse peritoneal macrophages with Rat anti Mouse CD1076 following permeabilsation with Leucoperm
  • FC - Anti-Mouse CD107b Antibody, clone M3/84  ABD12228
    Staining of mouse peritoneal macrophages with Alexa Fluor® 647 Rat anti MouseCD107b . Permeabilised with Leucoperm
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17047
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names M3/84
Calculated MW 45681 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Glycoproteins purified from mouse peritoneal macrophage membranes.
Shelf Life 18 months from date of despatch.
Gene ID 16784
Other Names Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B, CD107b, Lamp2, Lamp-2
Target/Specificity Rat anti-Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. Studies suggest that CD107b may be involved in leucocyte adhesion.The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD107b Antibody, clone M3/84 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Springer, T.A. (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.
J Biol Chem. 256 (8): 3833-9. 2. Flotte, T.J. et al. (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
Am J Pathol. 111 (1): 112-24. 3. Ho, M.K. & Springer, T.A. (1983) Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.
J Biol Chem. 258 (1): 636-42. 4. Ulrich, R. et al. (2010) Machine learning approach identifies new pathways associated with demyelination in a viral model of multiple sclerosis.
J Cell Mol Med. 14 (1-2): 434-48. 5. Amirbekian, V. et al. (2007) Detecting and assessing macrophages in vivo to evaluate atherosclerosis noninvasively using molecular MRI.
Proc Natl Acad Sci U S A. 104: 961-6. 6. Fan, D. et al. (2014) Differential role of TIMP2 and TIMP3 in cardiac hypertrophy, fibrosis, and diastolic dysfunction.
Cardiovasc Res. 103 (2): 268-80. 7. Higuchi, Y. et al. (2012) Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.
Am J Physiol Heart Circ Physiol. 302: H2352-62. 8. Ishibashi, M. et al. (2004) Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling.
Circ Res. 94: 1203-10. 9. Sato, A. et al. (2008) Thioredoxin-1 ameliorates cigarette smoke-induced lung inflammation and emphysema in mice.
J Pharmacol Exp Ther. 325: 380-8. 10. Xu, J. et al. (2007) Role of cardiac overexpression of ANG II in the regulation of cardiac function and remodeling postmyocardial infarction.
Am J Physiol Heart Circ Physiol. 293: H1900-7. 11. Zhao, Q. et al. (2004) Essential role of vascular endothelial growth factor in angiotensin II-induced vascular inflammation and remodeling.
Hypertension. 44: 264-70. 12. Raddatz, B.B. et al. (2016) Central Nervous System Demyelination and Remyelination is Independent from Systemic Cholesterol Level in Theiler's Murine Encephalomyelitis.
Brain Pathol. 26 (1): 102-19. 13. Herder, V. et al. (2015) Dynamic Changes of Microglia/Macrophage M1 and M2 Polarization in Theiler's Murine Encephalomyelitis.
Brain Pathol. 25 (6): 712-23. 14. Bröer S et al. (2016) Brain inflammation, neurodegeneration and seizure development following picornavirus infection markedly differ among virus and mouse strains and substrains.
Exp Neurol. pii: S0014-4886(16)30033-4.

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Cat# ABD12228
(40 western blots)
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