|Application ||WB, IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine alveolar macrophages|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a 155 kDa cell surface glycoprotein, member of the alpha integrin family. Clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001). CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets (Dominguezet al.2001).Mouse anti-Pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonised zymosan particles by polymononuclear granulocytes and alveolar macrophages (Bullidoet al.1996).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD11R3 Antibody, clone 2F4/11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bullido, R. et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis.J Immunol Methods 195: 125-134 2. Domingues, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.Vet Immunol Immunopathol. 80: 111-119 3. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection.Arch Virol. 148: 2307-2323 4. Van de Walle, G. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.J Gen Virol. 84: 629-637 5. Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.Xenotransplantation 7: 258-266 6. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.J Immunol. 162: 5230-7 7. Thorgersen, E.B. et al. (2010) Anti-inflammatory effects of C1-Inhibitor in porcine and human whole blood are independent of its protease inhibition activity.Innate Immun. 16: 254-64 8. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.FASEB J. 24: 712-22 10. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.J Immunol. 187: 4913-9 11. Jacobsen, M.J. (2015) Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in ObesityJournal of Diabetes Research Article ID 610546 [Epub ahead of print] 12. Crisci, E. et al. (2012) Chimeric calicivirus-like particles elicit specific immune responses in pigs.Vaccine. 30 (14): 2427-39. 13. Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.Eur J Dermatol. 23 (4): 456-66. 14. Baert K et al. (2015) Cell type-specific differences in β-glucan recognition and signalling in porcine innate immune cells.Dev Comp Immunol. 48 (1): 192-203. 15. Sbrana, s. et al. (2014) Phenotype Changes of Circulating Monocytes in a Hypercholesterolemic Swine Model of Coronary Artery DiseaseJournal of Cytology & Histology. 05 (05): .9. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54
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