|Application ||WB, IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine alveolar macrophages|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a 155 kDa cell surface glycoprotein, member of the alpha integrin family. Clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001). CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets (Dominguezet al.2001).Mouse anti-Pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonised zymosan particles by polymononuclear granulocytes and alveolar macrophages (Bullidoet al.1996).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD11R3 Antibody, clone 2F4/11 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bullido, R. et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis.J Immunol Methods 195: 125-134 2. Domingues, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.Vet Immunol Immunopathol. 80: 111-119 3. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection.Arch Virol. 148: 2307-2323 4. Van de Walle, G. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.J Gen Virol. 84: 629-637 5. Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.Xenotransplantation 7: 258-266 6. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.J Immunol. 162: 5230-7 7. Thorgersen, E.B. et al. (2010) Anti-inflammatory effects of C1-Inhibitor in porcine and human whole blood are independent of its protease inhibition activity.Innate Immun. 16: 254-64 8. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.FASEB J. 24: 712-22 10. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.J Immunol. 187: 4913-9 11. Jacobsen, M.J. (2015) Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in ObesityJournal of Diabetes Research Article ID 610546 [Epub ahead of print] 12. Crisci, E. et al. (2012) Chimeric calicivirus-like particles elicit specific immune responses in pigs.Vaccine. 30 (14): 2427-39. 13. Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.Eur J Dermatol. 23 (4): 456-66. 14. Baert K et al. (2015) Cell type-specific differences in β-glucan recognition and signalling in porcine innate immune cells.Dev Comp Immunol. 48 (1): 192-203. 15. Sbrana, s. et al. (2014) Phenotype Changes of Circulating Monocytes in a Hypercholesterolemic Swine Model of Coronary Artery DiseaseJournal of Cytology & Histology. 05 (05): .9. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54
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