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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig CD163 Antibody, clone 2A10/11 (RPE)   

Anti-Pig CD163 Antibody, clone 2A10/11 (FITC)

Mouse Anti-Pig Monoclonal Antibody

     
  • FC - Anti-Pig CD163 Antibody, clone 2A10/11 (FITC) ABD12237
    Published customer image:Mouse anti Pig CD163 antibody, clone 2A10/11 used for the detection of CD163 expressing cells in porcine synovium by immunohistochemistry on cryosections.Image caption:Light microscopy images of CD163 antibody stained histological sections obtained from the synovium (A, C, F, I, L), ligament (B, D, G, J, M), and provisional scaffold (E, H, K, N) at days 0 (A,B), 1 (C, D, E), 5 (F, G, H), 9 (I, J, K), and 14 (L, M, N) following ACL injury. Scale bar represents 50 μm.From: Haslauer CM, Proffen BL, Johnson VM, Hill A, Murray MM. Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model.J Inflamm (Lond). 2014 Nov 1;11:34.
  • IHC - Anti-Pig CD163 Antibody, clone 2A10/11 (FITC) ABD12237
    Published customer image:Representative pictures of immunohistochemical detection of CD163+ cells in tracheobronchial lymph node and spleen. CD163+ cells were detected in tracheobronchial lymph nodes from control (A) and APP-infected pigs (B) and in spleen from control (C) and APP-infected pigs (D). Immunohistochemical visualization: horseradish peroxidase, brown substrate, hematoxylin counterstain; c, cortex; ca, central artery; e, ellipsoid; f, follicle; mz, marginal zone; pals, periarterial lymphatic sheath, rp, red pulp; s, subcapsular sinus.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98.
  • FC - Anti-Pig CD163 Antibody, clone 2A10/11 (FITC) ABD12237
    Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163
  • FC - Anti-Pig CD163 Antibody, clone 2A10/11 (FITC) ABD12237
    Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 : FITC
  • FC - Anti-Pig CD163 Antibody, clone 2A10/11 (FITC) ABD12237
    Published customer image:Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/- SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession Q2VL90
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 2A10/11
Calculated MW 120456 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Porcine alveolar macrophages.
Shelf Life 18 months from date of despatch.
Gene ID 397031
Other Names Scavenger receptor cysteine-rich type 1 protein M130, CD163, Soluble CD163, sCD163, CD163, M130
Target/Specificity Mouse anti-Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a 120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchezet al.1999).Mouse anti-Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torreset al.2003).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig CD163 Antibody, clone 2A10/11 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Sanchez, M. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J. Immunol. 162: 5230-5237. 2. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocyte/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens 49: 403-413. 3. Yang, P. et al. (2002) Immune cells in the porcine retina: Distribution, characterization and morphological features.
Invest. Opthalmol. Vis. Sci. 43: 1488-1492. 4. Gomez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis
J. Virol. 73: 2173-2180. 5. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet. Immunol. Immunopathol. 80: 93-109. 6. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection.
Arch. Virol. 148: 2307-2323. 7. Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163.
BMC Biotechnol. 10: 48. 8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
Stem Cells. 26: 1347-55. 9. Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 41: 76. 10. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
Vet Res. 41: 64. 11. Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction.
Am J Physiol Heart Circ Physiol. 300: H693-701. 12. Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163.
J Virol. 84: 1731-40. 13. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9. 14. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94. 15. De Baere, M.I. et al. (2012) Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
Vet Res. 43: 47. 16. Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46. 17. Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
Acta Vet Scand. 56: 42. 18. Sang, Y. et al. (2014) Antiviral Regulation in Porcine Monocytic Cells at Different Activation States.
J Virol. pii: JVI.01714-14. 19. Haslauer, C.M. et al. (2014) Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model.
J Inflamm (Lond). 11 (1): 34.14. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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