|Calculated MW||120456 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Porcine alveolar macrophages.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Scavenger receptor cysteine-rich type 1 protein M130, CD163, Soluble CD163, sCD163, CD163, M130|
|Target/Specificity||Mouse anti-Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a 120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchezet al.1999).Mouse anti-Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torreset al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD163 Antibody, clone 2A10/11 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Sanchez, M. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.J. Immunol. 162: 5230-5237. 2. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocyte/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.Tissue Antigens 49: 403-413. 3. Yang, P. et al. (2002) Immune cells in the porcine retina: Distribution, characterization and morphological features.Invest. Opthalmol. Vis. Sci. 43: 1488-1492. 4. Gomez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesisJ. Virol. 73: 2173-2180. 5. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.Vet. Immunol. Immunopathol. 80: 93-109. 6. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection.Arch. Virol. 148: 2307-2323. 7. Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163.BMC Biotechnol. 10: 48. 8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.Stem Cells. 26: 1347-55. 9. Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.Vet Res. 41: 76. 10. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.Vet Res. 41: 64. 11. Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction.Am J Physiol Heart Circ Physiol. 300: H693-701. 12. Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163.J Virol. 84: 1731-40. 13. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.Vet Res. 42: 9. 14. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.J Immunol. 188: 3382-94. 15. De Baere, M.I. et al. (2012) Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.Vet Res. 43: 47. 16. Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.J Virol. 87: 9538-46. 17. Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.Acta Vet Scand. 56: 42. 18. Sang, Y. et al. (2014) Antiviral Regulation in Porcine Monocytic Cells at Different Activation States.J Virol. pii: JVI.01714-14. 19. Haslauer, C.M. et al. (2014) Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model.J Inflamm (Lond). 11 (1): 34.14. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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